G-Quadruplex DNA -

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Résumé :
Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In G-Quadruplex DNA: Methods and Protocols, experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology? series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, G-Quadruplex DNA: Methods and Protocols promises to be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.

Sommaire:
G-Quadruplexes: From Guanine Gels to Chemotherapeutics.- Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes.- Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential.- Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis.- Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay.- Real-Time Observation of G-Quadruplex Dynamics Using Single-Molecule FRET Microscopy.- Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures.- 2-Aminopurine as a Probe for Quadruplex Loop Structures.- Assessing DNA Structures with 125I Radioprobing.- Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques.- Probing Telomeric G-Quadruplex DNA Structures in Cells with In Vitro Generated Single-Chain Antibody Fragments.- Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC.- Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography.- Quantifying Interactions Between G-Quadruplex DNA and Transition-Metal Complexes.- G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands.

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