Alternative Splicing -

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Résumé :
Alternative Splicing in Human Biology and Disease.- Targeting Alternative Splicing for Therapeutic Interventions.- An Optimized Protocol for the Mapping of Cell Type-Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions.- FACS-Based Neuronal Cell Type-Specific RNA Isolation and Alternative Splicing Analysis.- Quantitative Measurement of Alternatively Spliced RNA Isoform Levels.- Analysis of Splicing Regulation by Third-Generation Sequencing.- Computational Analysis of Alternative Splicing Using VAST-TOOLS and the VastDB Framework.- Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data.- Functional Annotation of Custom Transcriptomes.- Design, Labeling, and Application of Probes for RNA smFISH.- BaseScope(TM) Approach to Visualize Alternative Splice Variants in Tissue.- In Situ Imaging of mRNA Splicing Variants by SpliceRCA.- Two-Color Fluorescent Reporters for Analysis of Alternative Splicing.- Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectrometry.- Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscopy.- Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention.- Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins.- Probing Liquid-Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo.- Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals....

Sommaire:
Alternative Splicing in Human Biology and Disease.- Targeting Alternative Splicing for Therapeutic Interventions.- An Optimized Protocol for the Mapping of Cell Type-Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions.- FACS-Based Neuronal Cell Type-Specific RNA Isolation and Alternative Splicing Analysis.- Quantitative Measurement of Alternatively Spliced RNA Isoform Levels.- Analysis of Splicing Regulation by Third-Generation Sequencing.- Computational Analysis of Alternative Splicing Using VAST-TOOLS and the VastDB Framework.- Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data.- Functional Annotation of Custom Transcriptomes.- Design, Labeling, and Application of Probes for RNA smFISH.- BaseScope(TM) Approach to Visualize Alternative Splice Variants in Tissue.- In Situ Imaging of mRNA Splicing Variants by SpliceRCA.- Two-Color Fluorescent Reporters for Analysis of Alternative Splicing.- Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectrometry.- Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscopy.- Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention.- Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins.- Probing Liquid-Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo.- Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals.