Neurotransmitter Methods - Richard C. Rayne

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Résumé :
This outstanding and comprehensive collection of user-friendly protocols is essential for neuroscientists who wish to localize, identify, purify, and/or quantify neurotransmitters. The easily reproducible methods presented cover a broad spectrum of approaches involving cell and organ culture, histochemistry, immunochemistry, in situ hybridization, cell fractionation, liquid chromatography, mass spectrometry, in vivo microdialysis, and voltammetry. These approaches are optimized for use by neuroscientists, are often complementary, and can be employed in tandem with powerful effect.BR

Sommaire:
Preparation of Brain Slices.- Preparation of Organotypic Hippocampal Slice Cultures Using the Membrane Filter Method.- The Preparation and Use of Brain Slices and Dissociated Neurons for Patch-Clamp Studies of Neurotransmitter Action.- Synaptosomes.- Synaptoneurosomes.- Preparation of Synaptosomal Plasma Membranes by Subcellular Fractionation.- Detection of Neuropeptides by Immunocytochemistry.- Immunocytochemical Detection of Amino Acid Neurotransmitters in Paraformaldehyde-Fixed Tissues.- cGMP-Immunocytochemistry.- Microwave Antigen Retrieval in Formaldehyde-Fixed Human Brain Tissue.- Localization of Nitric Oxide Synthase Using NADPH Diaphorase Histochemistry.- In Situ Hybridization to Determine the Expression of Peptide Neurotransmitters.- Quantitative In Situ Hybridization Histochemistry.- Microbore Liquid Chromatography Analysis of Monoamine Transmitters.- Microbore Liquid Chromatography Analysis of Amino Acid Transmitters.- HPLC Methods to Isolate Peptide Neurotransmitters.- Neuropeptide Expression Patterns as Determined by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry.- GC/MS Determination of Biogenic Amines in insect Neurons.- Monitoring Amino Acid Neurotransmitter Release in the Brain by In Vivo Microdialysis.- In Vivo Detection of Neurotransmitters with Fast Cyclic Voltammetry.