PCR Protocols in Molecular Toxicology - John P. van de Heuvel

. .

Résumé :
Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects.PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression, differential display cloning, cloning and library screening by PCR, genotype and polymorphism analysis of drug and toxicant metabolizing enzymes, basic, non-PCR based molecular biology methods. PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential. TOC:Basics of the Polymerase Chain Reaction, W.B. Mattes.- Analysis of Gene Expression, J.P. Vanden Heuvel.- Differential Display PCR, J.C. Corton.- Cloning by PCR, J.P. Vanden Heuvel.- Genotype Analysis, D.A. Bell and G. Pittman.- General Molecular Biology Techniques, J.P. Vanden Heuvel.- Appendix I: List of Suppliers.- Appendix II: Companies, Online Catalogs, and Internet Resources.- Index.