Allergy Methods and Protocols -

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Résumé :
Allergy: Methods and Protocols aims to assist the researcher in gaining insight into the molecular mechanisms involved in allergy by featuring an array of protocols. The protocols cover a range of disciplines including allergy, immunology, cell biology, and histology, and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, and T regulatory cells.

Sommaire:
List of chapters 1. Understanding the molecular mechanisms of allergy - Meinir Jones 2. T cell - primary culture from peripheral blood - Monika Raulf-Heimsoth 3. Production of T cell lines - Helga Kahlert 4. Production of human T cell clones - Adrienne Verhoef 5. Mapping of human T-cell epitopes of allergens - Thomas Zeiler and Tuomas Virtanen 6. Determining MHC restriction of T cell response - Mark Larche 7. Short term culture of CD8 cells and intracellular cytokine staining - Beejal Vyas and Alistair Noble 8. Isolation, flow cytometric analysis and suppression assay of CD4+CD25+ T regulatory cells - Hayley Jeal 9. Monocyte-derived dendritic cells as antigen presenting cells in T cell proliferation and cytokine production - Sun-sang J Sung 10. Ultrasensitive ELISA for measurement of human cytokine responses in primary culture - William P. Stefura, J. Darren Campbell, Ren?e Douville, Monique J. Stinson, F. Estelle Simons, Allan B. Becker, and Kent T. HayGlass 11. Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses - Monique Stinson, Renee Douville, Yuriy Lissitsyn, Melanie Blanchard, William Stefura, Estelle Simons, Allan Becker, Peter Nickerson, Kevin Coombs, Kent HayGlass 12. Standardisation of allergen extracts - J?rgen Nedergaard Larsen and Sten Dreborg 13. Immuno-electrophoresis for the characterisation of allergen extracts - Gitte Nordskov Hansen and J?rgen Nedergaard Larsen 14. Conjugation of haptens - Ranulfo Lemus and Meryl H. Karol 15. Monoclonal antibodies - Helga Kahlert and Oliver Cromwell 16. Purification of antibodies - Per H Larsson and Anne Renstr?m 17. Collection of air samples to quantify exposure to airborne allergens - Susan Gordon 18. Assay of air sample eluates - Anne Renstr?m and Susan Gordon 19. The halogen assay - a new technique for measuring airborne allergen - Euan Tovey,Sandra De Lucca, Leanne Poulos and Tim O'Meara 20. Measurement of specific IgG anti-Fel d 1 antibodies - Meinir Jones 21. The facilitated-antigen binding (FAB) assay - a protocol to measure allergen-specific inhibitory activity - James N Francis 22. Microscopic identification and purity determination of pollen grains - Magdalena Rahl 23. Biopanning for the characterisation of allergen mimotopes - Isabella Sch?ll and Erika Jensen-Jarolim 24. Identification of mast cells and mast cell subpopulations - Mark Buckley and Andrew F Walls 25. Purification and characterisation of mast cell tryptase and chymase - Alan R McEuen and Andrew F Walls 26. Experimental activation of mast cells and their pharmacological modulation - Shaoheng He and Andrew F Walls 27. In situ hybridisation - Kayhan T. Nouri-Aria