Hemoglobin Disorders -

. .

Résumé :
A collection of readily reproducible experimental and clinical techniques for studying the molecular basis of hemoglobin disorders. These step-by-step methods utilize a wide variety of the latest analytical techniques, including x-ray crystallography, high performance liquid chromatography, electrophoresis, and nuclear magnetic resonance. Additional methods are offered for prenatal diagnostic analysis, the DNA diagnosis of hemoglobin mutations, hemoglobin fluorescence, and the semisynthesis of hemoglobin.

Sommaire:
X-ray Crystallography of Hemoglobins Martin K. Safo and Donald J. Abraham Analysis of Hemoglobins and Globin Chains by High-Performance Liquid Chromatography Henri Wajcman Purification and Molecular Analysis of Hemoglobin by High-Performance Liquid Chromatograpy Belur N. Manjula and Seetharama A. Acharya Oxygen Equilibrium Measurements of Human Red Blood Cells Jean Kister and Henri Wajcman Measurement of Rate Constants for Reactions of O2, CO, and NO with Hemoglobin John S. Olson, Erin W. Foley, David H. Maillett, and Eden V. Paster Electrophoretic Methods for Study of Hemoglobins DNA Diagnosis of Hemoglobin Mutations John M. Old. Methods for Analysis of Prenatal Diagnosis, John M. Old Hemoglobin Fluorescence Rhoda Elison Hirsch Nucleation and Crystal Growth of Hemoglobins: The Case of HbC Peter G. Vekilov, Angela Feeling-Taylor, and Rhoda Elison Hirsch Semisynthesis of Hemoglobin Seetharama A. Acharya and Sonati Srinivasulu b-Globin-like Gene Cluster Haplotypes in Hemoglobinopathies Shanmugakonar Muralitharan, Rajagopal Krishnamoorthy, and Ronald L. Nagel Transgenic Mice and Hemoglobinopathies Mary E. Fabry, Eric E. Bouhassira, Sandra M. Suzuka, and Ronald L. Nagel Recombinant Single Globin-Chain Expression and Purification Kazuhiko Adachi Nuclear Magnetic Resonance of Hemoglobins Jonathan A. Lukin and Chien Ho Solubility Measurement of the Sickle Polymer Mary E. Fabry, Seetharama A. Acharya, Sandra M. Suzuka, and Ronald L. Nagel Index