Nitric Oxide Protocols -

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Résumé :
A collection of cutting-edge techniques for measuring nitric oxide and the enzyme that produces it in biological tissues and fluids. These readily reproducible methods can be used to measure novel nitric oxide-related products such as protein nitration and nitrosation, as well as to express nitric oxide synthase in basic research and gene therapy using viral vectors.

Sommaire:
Measurement of Nitric Oxide by Reconversion of Nitrate/Nitrite to NO Reinhard Berkels, Svenja Purol-Schnabel, and Renate Roesen High-Resolution Capillary Electrophoresis of Nitrite and Nitrate in Biological Samples Dmitri Y. Boudko Nitrite and Nitrate Measurements in Human Urine by Capillary Electrophoresis Edward Morcos and N. Peter Wiklund In Vivo Nitric Oxide Measurements Using a Microcoaxial Electrode Yoshiichiro Kitamura, Hirosuke Kobayashi, Kazuo Tanishita, and Kotaro Oka Measurement of Exhaled Nitric Oxide Peter A. Steerenberg and Jan G. C. van Amsterdam Nitric Oxide Imaging in Neurons Using Confocal Microscopy Xiaoxiang Zheng, Gangmin Ning, Dihui Hong, and Mu Zhang Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry Dimitrios Tsikas A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts Hazel Sutherland, Olga Zolle, Roba Khundkar, Alec W. M. Simpson, Jonathan C. Jarvis, and Stanley Salmons Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization Olivier Braissant Real-Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase Yi Chu and Frank M. Faraci Dioxygen-Dependent Metabolism of Nitric Oxide Paul R. Gardner, Anne M. Gardner, and Craig K. Hallstrom Proteomic Method for Identification of Tyrosine-Nitrated Proteins Kulwant S. Aulak, Thomas Koeck, John W. Crabb, and Dennis J. Stuehr Immunohistochemical Detection of S-Nitrosylated Proteins Andrew J. Gow, Christiana W. Davis, David Munson, and Harry Ischiropoulos Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernosa for Erectile Dysfunction Trinity J. Bivalacqua, Weiwen Deng, Hunter C. Champion, Wayne J. G. Hellstrom, and Philip J. Kadowitz Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer Into the Nucleus Tractus Solitarius in Conscious Rats Yoshitaka Hirooka and Koji Sakai Gene Therapy in Cancer Via Use of a Retrovector Having a Tumor Specificity and Expressing Inducible Nitric Oxide Synthase Masahide Kuroki Gene Transfer of Nitric Oxide Synthase Via the Use of Adeno-Associated Virus Vectors Yoshikazu Maeda, Kazuyuki Shimada, and Uichi Ikeda Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer Kathleen G. Raman, Richard A. Shapiro, Edith Tzeng, and Melina R. Kibbe Index