Steroid Receptor Methods -

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Résumé :
A distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA binding characteristics, hormone binding assays, and the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness.

Sommaire:
Part I. Bioinformatics Bioinformatics of Nuclear Receptors Mark Danielsen Phylogenetic Inference and Parsimony Analysis Llewellyn D. Densmore III Part II. Purification Protocols Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems Vida Senkus Melvin and Dean P. Edwards High-Yield Purification of Functionally Active Glucocorticoid Receptor Terace M. Fletcher, Barbour S. Warren, Christopher T. Baumann, and Gordon L. Hager Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor Mingmin Liao and Elizabeth M. Wilson Large Scale Production of Nuclear Receptor Ligand-Binding Domains Li-Zhi Mi and Fraydoon Rastinejad Physical Structure of Nuclear Receptor-DNA Complexes Scott A. Chasse and Fraydoon Rastinejad Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display Sushma Kumar, Maarit Angervo, Milan K. Bagchi, and Indrani C. Bagchi Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes Bruce Blumberg Part III. Steroid Hormone-Binding Assays Use of [99mTc] Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors Frank W?st Steroid Hormone Metabolites and Hormone Binding Assays Rosemary Bland and Martin Hewison Vitamin D3 Analog Screening Sami V?is?nen, Sanna Ryh?nen, and Pekka H. M?enp?? Part IV. Protein Interaction Assays Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors Steven K. Nordeen, Paul R. Housley, Yihong Wan, and Richard N. Day Knockout Mice and Steroid Receptor Research Per Flodby, Stephan Teglund, andJan-?ke Gustafsson Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors Bertrand Le Douarin, David M. Heery, Claudine Gaudon, Elmar vom Baur, and R?gine Losson Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex Rabindra N. Bhattacharjee, Caroline Underhill, and Joseph Torchia Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System Simon J. Evans and Frank L. Moore Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay James R. Lambert and Steven K. Nordeen Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcription In Vivo Christy J. Fryer and Trevor K. Archer Cotransfection Assays and Steroid Receptor Biology Shimin Zhang and Mark Danielsen Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System Hironobu Sasano, Sachiko Matsuzaki, and Takashi Suzuki Part V. Cancer Research and Drug Discovery Solid Tumor Cancer Markers and Applications to Steroid Hormone Research Marcia V. Fournier, Katherine J. Martin, and Arthur B. Pardee Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay Kathleen F. Arcaro and John F. Gierthy Combinatorial Chemistry in Steroid Receptor Drug Discovery John A. Flygare, Daniel P. Sutherlin, and S. David Brown Identification of Nuclear Receptor Interacting Proteins Using Yeast Two-Hybrid Technology: Applications to Drug Discovery Sunil Nagpal, Corine R. Ghosn, and Roshantha A. S. Chandraratna