Apoptosis Techniques and Protocols -

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Résumé :
In this revised and expanded second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of pro-apoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays.

Sommaire:
APOPTOSIS TECHNIQUES AND PROTOCOLS, 2ND EDITION Edited by: Andr?a C. LeBlanc CONTENTS Preface to the Series Preface Contributors Molecular Characterization of the Proapoptotic Protein Bax Yi-Te Hsu and Soraya Smaili Assessing Cytochrome-c Release from Mitochondria Douglas W. Ethell and Douglas R. Green Monitoring Caspases in Neuronal Cell Death Younes Bounhar, Omar Tounekti, and Andr?a C. LeBlanc HSV Amplicon Vectors in Neuronal Apoptosis Studies Kathleen A. Maguire-Zeiss, William J. Bowers, and Howard J. Federoff Microinjections to Study the Specific Role of Proapoptotic Proteins in Neurons Yan Zhang and Andr?a C. LeBlanc Designing Microarray Experiments for Neurobiology P. Scott Eastman and Jeanne F. Loring Analysis of Gene Expression by Nylon Membrane cDNA Arrays H. Michael Tucker and Steven Estus Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs Mark D. Berry and Paula C. Ashe Analytical Approaches for Investigating Apoptosis and Other Biochemical Events in Compartmented Cultures of Sympathetic Neurons Barbara Karten, Bronwyn L. MacInnis, Hubert Eng, Yoko Azumaya, Grace Martin, Karen Lund, Russell C. Watts, Jean E. Vance, Dennis E. Vance, and Robert B. Campenot Detection and Analysis of Synaptosis Greg M. Cole and Karen Gylys In Situ Detection of Apoptotic Neurons Kevin A. Roth Apoptotic and Oxidative Indicators in Alzheimer's Disease Arun K. Raina, Lawrence M. Sayre, Craig S. Atwood, Catherine A. Rottkamp, Ayala Hochman, Xiongwei Zhu, Mark E. Obrenovich, Shun Shimohama, Akihiko Nunomura, Atsushi Takeda, George Perry, and Mark A. Smith Index