Basic Cell Culture Protocols -

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Résumé :
In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

Sommaire:
Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line
Cheryl D. Helgason

Detection of Mycoplasma Contaminations
Cord C. Uphoff and Hans G. Drexler

Eradication of Mycoplasma Contaminations
Cord C. Uphoff and Hans G. Drexler

Authentication of Scientific Human Cell Lines: Easy-to-Use DNA Fingerprinting
Wilhelm G. Dirks and Hans G. Drexler

Cytogenetic Analysis of Cell Lines
Roderick A. F. MacLeod and Hans G. Drexler

Human and Mouse Hematopoietic Colony-Forming Cell Assays
Cindy L. Miller and Becky Lai

Isolation and Culture of Murine Macrophages
John Q. Davies and Siamon Gordon

Isolation and Culture of Human Macrophages
John Q. Davies and Siamon Gordon

Development of T-Lymphocytes in Mouse Fetal Thymus Organ Culture
Tomoo Ueno, Cunlan Liu, Takeshi Nitta, and Yousuke Takahama

In Vitro Generation of Lymphocytes From Embryonic Stem Cells
Ren?e F. de Pooter, Sarah K. Cho, and Juan Carlos Z??iga-Pfl?cker

Hematopoietic Development of Human Embryonic Stem Cells in Culture
Xinghui Tian and Dan S. Kaufman

Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aorta-Gonads-Mesonephros Region
Robert A. J. Oostendorp, Kirsty Harvey, and Elaine A. Dzierzak

Culture of Human and Mouse Mesenchymal Cells
Emer Clarke

Isolation, Purification, and Cultivation of Murine and Human Keratinocytes
Frizell L. Vaughan and Ludmila I. Bernstam

Isolation and Culture of Primary Human Hepatocytes
Edward L. LeCluyse, Eliane Alexandre, Geraldine A. Hamilton, Catherine Viollon-Abadie, D. James Coon, Summer Jolley, and Lysiane Richert

Primary Kidney Proximal Tubule Cells
Mary Taub

Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect ProgenitorActivity
John Stingl, Joanne T. Emerman, and Connie J. Eaves

Generation and Differentiation of Neurospheres From Murine Embryonic Day 14 Central Nervous System Tissue
Sharon A. Louis and Brent A. Reynolds

Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells
Gabi Shefer and Zipora Yablonka-Reuveni

Adult Ventricular Cardiomyocytes: Isolation and Culture
Klaus-Dieter Schl?ter and Daniela Schreiber

Isolation and Culture of Primary Endothelial Cells
Bruno Larriv?e and Aly Karsan

Studying Leukocyte Rolling and Adhesion In Vitro Under Flow Conditions
Susan L. Cuvelier and Kamala D. Patel

Isolation and Characterization of Side Population Cells
Margaret A. Goodell, Shannon McKinney-Freeman, and Fernando D. Camargo

Scalable Production of Embryonic Stem Cell-Derived Cells
Stephen M. Dang and Peter W. Zandstra

Index