Mycobacteria Protocols - Tanya Parish

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Résumé :
Mycobacteria include species of great medical importance, being - sponsible for both human and animal disease, including tuberculosis and lep- . The use of molecular techniques to explore the biology of these organisms rosy has exploded in the past few years. Mycobacteria pose particular problems in the laboratory, most notably their slow growth rate, their tendency to clump, and their resistance to standard methods for lysis; thus special adaptations of provides - commonly used methods are required . Mycobacteria Protocols scriptions of both basic and recently developed techniques for studying the molecular biology of mycobacteria. Although many genetic tools are still - quired, there is much that can be accomplished using those available, and this book provides a starting point for anyone interested in working on these - triguing, but difficult bacteria . The first eight chapters describe the basic techniques required for any study of mycobacteria, including culture and safety aspects, and preparation of nucleic acids, proteins, and cell-wall fractions. The introduction of DNA into mycobacteria was a major advance in enabling the development of - netic systems. Two chapters deal with introduction of DNA by electroporation and conjugation. Phages have also been used as the basis for genetic systems, . and a comprehensive list of protocols for their use is presented It is becoming difficult to handle the rapidly increasing body of kno- edge in the mycobacterial field.

Sommaire:
Mycobacteria: Bugs and Bugbears Tanya Parish and Neil G. Stoker Mycobacteria: General Culture Methodology and Safety Considerations Bryan W. Allen Isolation of Genomic DNA from Mycobacteria John T. Belisle and Michael G. Sonnenberg Nucleic-Acid Extraction: Plasmid/Cosmid DNA Bonnie B. Plikaytis and C. Harold King Pulsed Field Gel Electrophoresis for Mycobacteria Wolfgang J. Philipp,Stephen Gordon, Amalio Telenti, and Stewart T. Cole Extraction of RNA from Mycobacteria Eshwar Mahenthiralingam Preparation of Cell-Free Extracts from Mycobacteria Tanya Parish and Paul R. Wheeler Preparation of Cell-Wall Fractions from Mycobacteria Gurdyal S. Besra Using MycDB on the World-Wide Web Staffan Bergh and Stewart T. Cole Conjugating DNA into Mycobacteria Julie-Ann Gavigan and Carlos Martin Electroporation of Mycobacteria Mycobacteriophages Gary J. Sarkis and Graham F. Hatfull Chemical Mutagenesis of Mycobacteria Lucy A. Brooks Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System Esther P?rez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte Giguel, and Carlos Martin Homologous Recombination in Mycobacterium smegmatis: Screening Methods for Detection of Gene Replacement Robert N. Husson Gene Replacement in Mycobacterium smegmatis Using a Dominant Negative Selectable Marker Peter Sander and Erik C. BÖttger Gene Replacement in Mycobacterium intracellulare Britt-Inger Marklund and Richard W. Stokes Gene Replacement in Mycobacterium bovis BCG Elizabeth Norman The Application of Bacterial Luciferase as a Reporter Gene in Mycobacteria Stephen Gordon, Gavin Chung, and PeterAndrew Green Fluorescent Protein: A Tool for Gene Expression and Cell Biology in Mycobacteria Laura E. Via, Subramanian Dhandayuthapani, Dusanka Deretic, and V. Deretic Expression of Genes in Mycobacteria Peadar - Gaora Secretion of Mammalian Proteins from Mycobacteria Peter J. Murray and Richard A. Young Intracellular Gene Expression: Analysis of RNA from Mycobacteria in Macrophages Using RT-PCR Philip D. Butcher, Joseph A. Mangan, and Irene M. Monahan Analysis of Mycobacterial Differential Gene Expression by RAP-PCR Joseph A. Mangan and Philip D. Butcher Mycobacterial Speciation Francis A. Drobniewski and Anne H. C. Uttley Species Identification of Mycobacteria Using rDNA Sequencing Philip Kirschner and Erik C. BÖttger Detection of Mycobacterium tuberculosis by a Colorimetric Polymerase Chain Reaction Stuart M. Wilson Rapid Detection and Simultaneous Strain Differentiation of Mycobacterium tuberculosis Complex Bacteria by Spoligotyping Henri O. F. Molhuizen, Annelies E. Bunschoten, Leo M. Schouls, and Jan D. A. van Embden Computer Analysis of IS6110 RFLP Patterns of Mycobacterium tuberculosis Herre F. Heersma, Kristin Kremer, and Jan D. A. van Embden Detection of Mutations in Mycobacteria by PCR-SSCP(Single-Strand Conformation Polymorphism) Amalio Telenti, Nadine Honor?, and Stewart T. Cole The Use of Luciferase-Reporter Phage for Antibody-Specific Testing of Mycobacteria Paul F. Riska and William R. Jacobs, Jr Index