Correlation Force Spectroscopy for Single Molecule Measurements - Radiom, Milad

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Résumé :
This thesis?addresses the development of a new force spectroscopy tool, correlation force spectroscopy (CFS) for the measurement of the properties of very small volumes of material (molecular to ?m³) at kHz-MHz frequency range. CFS measures the simultaneous thermal fluctuations of two closely-spaced atomic force microscopy (AFM) cantilevers.? CFS then calculates the cross-correlation in the thermal fluctuations that gives the mechanical properties of the matter that spans the gap of the two cantilevers. The book also discusses development of CFS, its advantages over AFM, and its application in single molecule force spectroscopy and micro-rheology.

Biographie:
Milad Radiom received his PhD in Chemical Engineering at Virginia Tech in 2014, after MEng and BSc in Thermal and Fluids Engineering and Mechanical Engineering respectively from Nanyang Technological University and Amirkabir University of Technology. Thereafter, he was appointed as a postdoctoral research associate in Laboratory of Colloid and Surface Chemistry, University of Geneva.? His research interests are physical chemistry of polymers, colloids and surfaces as related to single molecule force spectroscopy, micro-rheology and surface forces....

Sommaire:

Chapter 1 - Introduction Overview of Single Molecule Force Spectroscopy Techniques Experiments Using Single molecule AFM Limitations of AFM-based single molecule force spectroscopy Summary?

Chapter 2- Correlation Force Spectroscopy? Correlation Force Spectroscopy: Rationale? Correlation Force Spectroscopy: Development Laterally Offset Configuration? Vertically Offset Configuration? Analysis of Correlations between Two Cantilevers? Validation of Fluctuation-Dissipation Theorem for One Cantilever? Analysis of Thermal Fluctuations to obtain Correlations?

Chapter 3- Dynamics of Single Molecules? Elastic Properties of Single Molecules: Worm-Like Chain and Freely-Jointed Chain Models Hydrodynamics of Single Molecules: Dumbbell Model and Rouse Model Internal Friction Rouse with Internal Friction Model of Linear Viscoelasticity of a Semiflexible Chain Summary

Chapter 4- Microrheology with Correlation Force Spectroscopy Existing Techniques of Rheometry Experimental Methods Comparison to Finite Element Analysis Comparison to Simple Harmonic Oscillator Model Summary

Chapter 5- Development of Colloidal Probe Correlation Force Spectroscopy: Case Study Materials and Methods Analysis Results? Discussion? Summary?

Chapter 6- Correlation Force Spectroscopy for Single Molecule Measurements Effect of the Distance between Cantilever Tips Harmonic Oscillator Modeling of Vertically Offset Correlation Force Spectroscopy Summary

Chapter 7- Single Molecule Force Spectroscopy of Dextran Results Stretching a Dextran Molecule Static Force-Elongation Mode Dynamic Correlated Fluctuations Mode Discussion Summary?

Chapter 8- Single Molecule Force Spectroscopy of Single-Stranded DNA Rationale? Introduction?Materials and Methods? Modal Analysis Summary

Chapter 9- Summary An Overview of Chapters? Future Work

Appendix I: Simple Harmonic Oscillator Model for Laterally Offset Correlation Force Spectroscopy

Appendix II: Simple Harmonic Oscillator Model for Vertically Offset Correlation Force Spectroscopy with Tethered Molecule

References