Vascular Morphogenesis -

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Résumé :
Historical Overview of the In Vivo and In Vitro Angiogenesis Assays.- The Fundamental Contribution of Judah Folkman in the Setting of Angiogenesis Assays.- Identification of Endothelial Cells and Their Progenitors.- In Vitro Co-Culture Assays of Angiogenesis.- Endothelial Cells: Co-Culture Spheroids.- Microfluidic Device Setting by Co-Culturing Endothelial Cells and Mesenchymal Stem Cells.- Spatial Statistics-Based Image Analysis Methods for the Study of Vascular Morphogenesis.- Studying Angiogenesis in the Rabbit Corneal Pocket Assay.- Avians as a Model System of Vascular Development.- Dynamic Imaging of Mouse Embryos and Cardiac Development in Static Culture.- High-Resolution Confocal Imaging of Pericytes in Human Fetal Brain Microvessels.- Quantification of Tumor Vasculature by Analysis of Amount and Spatial Dispersion of Caliber-Classified Vessels.- A Xenograft Model for Venous Malformation.- In Vivo Vascular Network Forming Assay.- Assessment of Vascular Patterning in the Zebrafish.- By the Skin of Your Teeth: A Subcutaneous Mouse Model to Study Pulp Regeneration....

Sommaire:
This detailed new edition aims to provide a range of methods and protocols for studying vascular morphogenesis in vivo and in vitro to reflect up-to-date advances in the field. Of note, the book explores endothelial cell signaling is currently understood to promote fundamental cues for cell fate specification, embryo patterning, organ differentiation, and postnatal tissue remodeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.