Protocols in Actinobacterial Research - Balagurunathan, Ramasamy

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Résumé :
This comprehensive laboratory manual describes the various protocols involved in Actinobacterial research. The content is divided into fifteen major sections, each of which is further divided into sub-sections describing the respective aim, principles, materials & methods, protocol, expected results and diagrams. Readers will find essential protocols for e.g. sample collection, isolation, characterization, analysis, profiling and evaluation of Actinobacteria for various applications. Gathering all relevant protocols concerning Actinobacteria, and written by a team of experienced Actinobacterial researchers, it is the first book of its kind. ...

Biographie:
Dr. Ramasamy Balagurunathan has been working for the past three decades exclusively in actinobacteria research for production of bioactive compounds. He has obtained his Doctoral degree in Marine Biology from Annamalai University for his research on bioactive compounds from marine Streptomyces. He pursued his post doctoral research on rare actinobacteria in reputed research Institutes in China and Malaysia. In 2019, he has obtained Doctor of Science degree in Microbiology from Periyar University for his research contribution on Bioprospecting of actinobacteria from rare ecosystems in India. He started his teaching career in 2000. He has handled eleven research projects on actinobacteria worth around Rs. 2.5 Crore. His research team discovered novel anti-TB and anti-HIV molecule from marine Streptomyces sp. R2 and named as TRANSITMYCIN. He is the Co-inventor in the US & Indian patent filed on Transitmycin. He has isolated Leptomycin - an anti-leptospiral compound from marine actinobacteria. With the goal of developing a centre for collection of actinobacteria from less explored habitats such as marine, desert, forest and mountain ecosystems, presently his team has isolated and are maintaining more than 500 actinobacteria isolated from various extreme environments including Thar desert, Himalayas, western Ghats, rubber forest, Andaman & Nicobar islands, which are the sources for production of antibiotics and other high value products. He has authored 145 publications including research articles, reviews, books, book chapters and magazine articles around the single focused aspect of actinobacteria for various applications like bioactive compound and bio-nanotechnology. He has an H index 19 and I10-index 28. He has guided 80 M. Sc., 25 M. Phil. Projects, 13 Ph.D thesis awarded and is guiding 6 Ph.D., scholars and 1 Post-doctoral fellow. Dr. Manikkam Radhakrishnan is a microbiologist pursuing research on Ecology, Systematics and Bioprospecting of actinobacteria from under studied ecosystems across India for the past two decades. He obtained his Doctoral degree in Microbiology from National Institute for Research in Tuberculosis under University of Madras on Antitubercular metabolites from Desert soil Streptomyces. After his Post graduation, he started his career as Assistant Professor in Microbiology. He is passionate about research and joined at Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology in the year 2013. He set up a consortium of >500 actinobacterial cultures under his headship in Bioprospecting division. He has isolated a novel chromopeptide molecule, Transitmycin which is effective against TB and HIV, from a novel marine Streptomyces species R2 isolated from Coral reef ecosystem of South India.. At present he is working on Diversity and Taxonomy of Psychrophilic and psychrotolerant actinobacteria from Indian sector of Southern Ocean (Antarctic). He is participating as the scientific member (Microbiology) in the 11^th Indian Scientific Expedition to Southern Ocean. He has authored 110 publications including research articles, reviews, book chapters and magazine articles and has also three patents on actinobacteria to his credit. It is his vision that this gold mine of Actinobacterial resource should be best exploited for the welfare of humans, animals, aquaculture and environmental applications since it has proven therapeutic potential and translational scope. Dr. Thangavel Shanmugasundaram has completed his Masters in Microbiology with gold medal from Periyar University. He has received prestigious INSPIRE fellowship from DST-SERB to pursue his doctoral degree on Exploring magnesite soil actinobacteria for metal and metal alloy nanoparticles for its biomedical and environment...

Sommaire:
Chapter 1. Sample collection, isolation and diversity of actinobacteria.- Chapter 2. Dereplication and ex situ conservation of actinobacteria.- Chapter 3. Characterization and identification of actinobacteria.- Chapter 4. Screening of actinobacteria for biological activities.- Chapter 5. Production of bioproducts from actinobacteria.- Chapter 6. Eliciting cryptic metabolite production from actinobacteria.- Chapter 7. Metabolite profiling of actinobacterial metabolites.- Chapter 8. Bioassay guided isolation and characterization of metabolites from actinobacteria.- Chapter 9. Evaluation of actinobacteria for environmental applications.- Chapter 10. Evaluation of actinobacteria for agricultural applications.- Chapter 11. Evaluation of actinobacteria for aquaculture applications.- Chapter 12. Evaluation of actinobacteria for nanoparticle synthesis.- Chapter 13. Determination of cytotoxicity of actinobacterial extracts & metabolites.- Chapter 14. Evaluation of actinobacteria for bio energyapplications.