Forensic DNA Typing Protocols -

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Résumé :
A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

Sommaire:
Forensic DNA-Typing Technologies: A Review Angel Carracedo and Paula S?nchez-Diz DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR Silvano K?chl, Harald Niederst?tter, and Walther Parson A Real-Time PCR Protocol to Determine the Number of Amelogenine (X-Y) Gene Copies From Forensic DNA Samples Antonio Alonso and Pablo Mart?n Species Determination: The Role and Use of the Cytochrome b Gene Adrian Linacre and James Chun-I Lee Constructing STR Mulitplex Assays John M. Butler Y Chromosome STR Typing Leonor Gusm?o and C?ntia Alves Using Online Databases for Developing SNP Markers of Forensic Interest Christopher Phillips SNP Typing in Forensic Genetics: A Review Beatriz Sobrino and Angel Carracedo Genotyping SNPs With the LightCycler Mar?a Victoria Lareu and Clara Ruiz-Ponte Universal Tag Arrays in Forensic SNP Analysis Marie Allen and Anna-Maria Divne SNP Typing on the NanoChip Electronic Microarray Claus B?rsting, Juan J. Sanchez, and Niels Morling Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS Peter M. Vallone, Kristina Fahr, and Markus Kostrzewa Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing Marie Allen and Hanna Andr?asson SNaPshot Typing of Mitochondrial DNA Coding Region Variants Antonio Salas, Beatriz Quint?ns, and Vanesa ?lvarez-Iglesias Typing of Y Chromosome SNPs With Multiplex PCR Methods Juan J. Sanchez, Claus B?rsting, and Niels Morling Y Chromosome SNP Analysis Using Single-Base Extension: A Hierarchical Multiplex Design Mar?a Bri?n SNaPshot for Pharmacogenetics by Minisequencing Klaus Bender Methods for the Study of Ancient DNA Christine Keyser-Tracqui and BertrandLudes Protocols for Ancient DNATyping Cristian Capelli and Frank Tschentscher Index