Stem Cell Protocols -

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Résumé :
This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ? Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.

Sommaire:

A Short Primer in Stem Cell Biology.- Measurement of Hematopoietic Stem Cell Proliferation, Self-Renewal and Expansion Potential.- Measuring the Aging Process in Stem Cells.- Measuring the Potency of a Stem Cell Therapeutic.- Culturing Protocols for Human Multipotent Adult Stem Cells.- The Isolation of Murine Bone Marrow Scavenging Sinusoidal Endothelial Cells.- Analysis of Circadian Rhythms in Embryonic Stem Cells.- Measuring Stem Cell Circadian Rhythms.- Cryopreservation of Human Pluripotent Stem Cells: A General Protocol.- Biological Differences Between Native and Cultured Mesenchymal Stem Cells - Implications for Therapies.- The Use of Multiparameter Flow Cytometry and Cell Sorting to Characterize Native Human Bone Marrow Mesenchymal Stem Cells (MSC).- High Yield Recovery of Equine MEsenchymal Stem Cells from Umbilical Cord Matrix/Wharton's Jelly Using a Semi-Automated Process.- Isolation and Functional Assessment of Cutaneous Stem Cells.- Isolation of Adult Stem Cell Populations from the Human Cornea.- Advanced Imaging and Tissue Engineering of the Human Limbal Epithelial Stem Cell Niche.- Isolation and Characterization of Stem Cells in the Adult Mammalian Ovary.- Clonal Culture of Adult Mouse Lung Epithelial Stem/Progenitor Cells.- Culture and Characterization of Mammary Cancer Stem Cells in Mammospeheres.- Isolation and Culture of Primary Glioblastoma Cells from Human Tumor Specimens.