Pediatric Hematology -

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Résumé :
Molecular techniques are now an integral part of the practice of modern pediatric hematology. In Pediatric Hematology: Methods and Protocols, hands-on masters of laboratory techniques describe their best methods for investigating and detecting a wide variety of hematological disorders. Here, the reader will find cutting-edge molecular protocols for the diagnosis of Fanconi anemia, dyskeratosis congenita, immunodeficiency, and most forms of hemoglobinopathy. In addition, there are detailed methods for molecular human platelet antigen genotyping, an effective PCR procedure for thrombophilia screening, and protocols for fluorescent in situ hybridization for cases in which insufficient metaphases are recovered or cryptic translocations are not visible. Since the measurement of minimal residual disease (MRD) provides a much more accurate risk-directed therapy, three methods are presented for detecting residual leukemia below the threshold of light microscopy, along with relatively simple, rapid, and inexpensive methods for the detection of MRD in ALL and AML. On the cutting-edge of technology, microarrays are used for the analysis of gene expression in childhood leukemia, and novel methods of graft engineering and reduced intensity conditioning regimens are applied to tissue typing for the assessment of donor/recipient chimerism. Each readily reproducible protocol is described in step-by-step detail and includes an introduction to the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Pediatric Hematology: Methods and Protocols offers experimental and clinical investigators a gold-standard collection of reliable molecular techniques essential to the diagnosis, classification, and treatment of childhood hematological disorders.

Sommaire:
Bone Marrow Failure Syndromes.- Molecular Diagnosis of Fanconi Anemia and Dyskeratosis Congenita.- Molecular Diagnosis of Diamond-Blackfan Anemia.- Red Blood Cell Disorders.- Antenatal Diagnosis of Hemoglobinopathies.- Platelet and Hemostatic Disorders.- Prenatal Diagnosis of Hemophilia.- Human Platelet Antigen Genotyping by PCR-SSP in Neonatal/Fetal Alloimmune Thrombocytopenia.- Multiplex PCR for the Detection of the Factor V Leiden and Prothrombin 20210A Mutations.- Immunodeficiency.- Molecular Diagnosis of Congenital Immunodeficiency.- Acute Leukemia.- Molecular Techniques to Improve Outcome in Childhood ALL.- Molecular Cytogenetics in Childhood Leukemia.- Fluorescent IgH Fingerprinting to Assess Minimal Residual Disease in Childhood B-Lineage ALL.- Real-Time Quantitative RT-PCR to Detect Fusion Gene Transcripts Associated With AML.- Real-Time PCR to Detect Minimal Residual Disease in Childhood ALL.- Oligonucleotide Microarray Analysis of Gene Expression in Leukemia.- Bone Marrow Transplantation.- HLA Low-Resolution Genotyping for Hematopoietic Stem Cell Transplantation.- DNA Sequencing as a Tissue-Typing Tool.- Analysis of Chimerism After Stem Cell Transplantation.