RNA Interference -

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Résumé :
RNA Interference: Challenges and Therapeutic Opportunities provides readers with recent advances in siRNA design, delivery, targeting and methods to minimize siRNA?s unwanted effects. Preclinical and clinical use of synthetic siRNAs, the roles of miRNAs in cancer and the promise of extracellular miRNAs for diagnosis are also covered in this meticulous collection, along with novel methods for identifying endogenous siRNAs and the annotation of small RNA transcriptomes. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.Comprehensive and cutting-edge, RNA Interference: Challenges and Therapeutic Opportunities will aid researchers, clinicians, teachers and biotechnologists interested in the power of RNA-based therapies.

Sommaire:

RNA Interference: Mechanisms, Technical Challenges and Therapeutic Opportunities.- Nanostructured RNAs for RNA Interference.- One Long Oligonucleotide or Two Short Oligonucleotides Based shRNA Construction and Expression.- PLGA Microspheres Encapsulating siRNA.- Magnetic Nanoparticle and Magnetic Field Assisted siRNA Delivery In Vitro.- Cytoplasmic Delivery of siRNAs to Monocytes and Dendritic Cells Via Electroporation.- Delivery of siRNAs to Cancer Cells Via Bacteria.- Microwell Array-Mediated Delivery of Lipoplexes Containing Nucleic Acids for Enhanced Therapeutic Efficacy.- Modulating the Tumor Microenvironment with RNA Interference as a Cancer Treatment Strategy.- Targeted In Vivo Delivery of siRNA and an Endosome-Releasing Agent to Hepatocytes.- Cell-Internalization SELEX: Method for Identifying Cell-Internalizing RNA Aptamers for Delivering siRNAs to Target Cells.- Strategies for siRNA Navigation to Desired Cells.- Analysis of RNA Interference-Mediated Hepatic Gene Silencing Following Murine Hydrodynamic Injection of Guanidinopropyl-Modified siRNAs.- Therapy of Respiratory Viral Infections with Intranasal siRNAs.- Engineering Therapeutic Cancer Vaccines that Activate Anti-Tumor Immunity.- Immunosuppressive Factor Blockade in Dendritic Cells Via siRNAs Results in Objective Clinical Responses.- Targeting bcr-abl Transcripts with siRNAs in an Imatinib-Resistant Chronic Myeloid Leukemia Patient: Challenges and Future Directions.- A Facile Method for Interfering with Off-Target Silencing Mediated by the Sense Strand.- Overcoming the Challenges of siRNA Activation of Innate Immunity: Design Better Therapeutic siRNAs.- Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs.- In Silico Identification of Novel Endo-siRNAs.- Computer-Assisted Annotation of Small RNA Transcriptomes.- miR-CATCH: MicroRNA Capture Affinity Technology.- Roles of MicroRNAs in Cancers and Development.- Targeting MicroRNAs to Withstand Cancer Metastasis.- Urinary MicroRNAsas a New Class of Non-Invasive Biomarkers in Oncology, Nephrology and Cardiology.- Magnetic Bead-Based Isolation of Exosomes.- Modified 2'-Ribose Small RNAs Function as Toll-Like Receptor-7/8 Antagonists.