Methods in Protein Sequence Analysis - Höög

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Résumé :
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Sommaire:
Sequencer Methodology and Instrumentation.- Modular Berlin Microsequencer for the Sequential Degradation of Proteins and Peptides from the Amino- and Carboxyl-Terminal End.- C-Terminal Sequence Analysis.- Chemical C-Terminal Sequencing.- Extending the Performance of the Solid-Phase Protein Sequencer.- Direct Microsequencing of Blotted and Covalently Attached Proteins in a Cross-Flow Reaction Chamber.- Sample Preparation and Analysis.- Current Strategies for Microscale Purification of Protein and Peptides for Sequence Analysis.- Capillary Electrophoresis: A New Dimension in the Separation Sciences.- Structural Analysis of Membrane Proteins.- Protein-Electroblotting and Microsequencing in Establishing Integrated Human Protein Databases.- Comparison of the Blotting Efficiencies of Various PVDF Membranes.- Sensitive Determination of Amino Acid Derivatives from N-Terminal Sequence Analysis.- Amino Acid Analysis and Sequencing - What is State-of-the-Art?.- Realistic Expectations for Amino Acid Analysis.- Modified Residues, Chemical Problems and Synthetic Peptides.- A Protein Chemistry Approach to the Modelling of Integral Membrane Proteins.- N-Terminal Acetylated Mitochondrial Aldehyde Dehydrogenase is Found in Fresh but not Frozen Liver Tissue.- Elucidating Ligand Binding Sites in Polypeptides by Photoaffinity Labeling with Aryl Azides.- Use of Thiopropyl-Sepharose 6B for Isolation and Structure-Functional Analysis of Thiol Proteins.- Zinc Fingers Involved in MHC Class I Gene Regulation: Use of Synthetic Peptides for Structural Analysis.- Hydrophobic Surfactant Proteins SP-B and SP-C: Special Analytical Problems.- Proteolysis.- The Yeast Prohormone-Processing Kex2 Protease, an Enzyme with Specificity for Paired Basic Residues.- Structures of Three Inhibitor Complexes of HIV-1 Protease.- Protease Specificity and Protein Sequence Analysis.- Cleavage-Sites in Protein Targeting Signals.- Studies on a Dimeric Aspartic Protease from a Single Domain of Pepsin.- Mass Spectrometry.- LC/MS and LC/MS/MS Screening for the Sites of Post-Translational Modification in Proteins.- Protein and Peptide Sequence Analysis by Tandem Mass Spectrometry in Combination with Either Capillary Electrophoresis or Micro-Capillary HPLC.- Plasma Desorption Mass Spectrometry as a Tool for Characterization of Native and Modified Forms of Recombinant Polypeptides.- Plasma Desorption Mass Spectrometry in Monitoring Peptide Synthesis and Phosphorylation Reactions.- Synergism with DNA Analysis.- Repeating Domains in the Plasma Proteins Participating in Blood Coagulation and Fibrinolysis.- Structural Analysis of the Glucocorticoid Receptor Protein.- C1? Inhibitor: Structure, Genetic Variants and Serpin Homologies.- Genetic Strategies for Protein Purification.- Predictions, Data Banks, Patterns and Tertiary Structures.- The Prediction of the Secondary Structure of Proteins.- A Computer Method of Finding Supersecondary Structures.- Usefulness of the PIR Database for Protein Comparisons.- The Structure and Post-Translational Modification of Lipoyl Domains in 2-Oxo Acid Dehydrogenase Multienzyme Complexes.- Zinc Chemistry in Function and Structure of Zinc Proteins.- Patterns of Sequence Variation in Families of Homologous Proteins.- Protein Folding: Local Structures, Domains and Assemblies.