Immunology in Plant Sciences -

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Sommaire:
Plant Hormone Immunoassays Based on Monoclonal and Polyclonal Antibodies.- 1 Introduction.- 2 Synthesis of Immunogens.- 3 Radio- and Enzyme-Labeling of Plant Hormones.- 4 Immunological Procedures.- 5 Antiserum Processing.- 6 Production of Monoclonal Antibodies.- 7 Radioimmunoassay Procedures.- 8 Enzyme Immunoassays Based on Monoclonal Antibodies.- 9 Plant Immunoanalysis.- References.- Radioimmunoassay and Gas Chromatography/Mass Spectrometry for Cytokinin Determination.- 1 Introduction.- 2 Theoretical Considerations for Analytical Methods.- 3 Radioimmunoassay.- 4 Gas Chromatography/Mass Spectrometry.- 5 Concluding Remarks.- References.- Immunodetection of Phytochrome: Immunocytochemistry, Immunoblotting, and Immunoquantitation.- 1 Introduction.- 2 Immunocytochemistry.- 3 Immunoblotting.- 4 Immunoquantitation.- 5 Concluding Remarks.- References.- Radioimmunoassay for a Soybean Phytoalexin.- 1 Isolation of Glyceollin I.- 2 Synthesis of Glyceollin I-BSA Conjugate.- 3 Generation of Antisera.- 4 Preparation of the [125I]-Glyceollin I Tracer.- 5 Radioimmunoassay for Glyceollin I.- 6 Specificity of the Radioimmunoassay.- 7 Application of the Radioimmunoassay for Glyceollin I to the Quantitation of Phytoalexins in Infected Soybean Tissue.- References.- The Measurement of Low-Molecular-Weight, Non-Immunogenic Compounds by Immunoassay.- 1 Introduction.- 2 Development of an Immunoassay.- 3 Immmunoassays.- Appendix: Sources of Materials and Equipment for ELISA.- References.- Radioimmunoassay and Western Blot Analysis of Acyl Carrier Protein Isoforms in Plants.- 1 Introduction.- 2 Production of ACP Antibody.- 3 Radioimmunoassay.- 4 Immunological Analysis of ACP Isoforms.- 5 Conclusion.- References.- Immunofluorescent Labelling of Enzymes.- 1 Introduction.- 2 General Methodology.- 2.5 Controls.- 3 Examples for Localization of Enzymes in Plant Tissue by Immunofluorescent Labeling.- References.- Quantitative Immunochemistry of Plant Phosphoenolpyruvate Carboxylases.- 1 Purificationof PEPC - Preparation of Immune Sera.- 2 Quantitative Immunoprecipitation in Gels.- 3 Immunotitration Coupled to Enzyme Activity.- 4 Quantitative Immunoprecipitation in Extracts.- 5 Immunoaffinity Chromatography.- 6 Concluding Remarks.- References.- Immunochemical Methods for Higher Plant Nitrate Reductase.- 1 Introduction.- 2 Nitrate Reductase Purification.- 3 Production of Nitrate Reductase Antiserum.- 4 Characterization of Nitrate Reductase Antiserum.- 5 Applications.- 6 Summary.- References.- Immunological-Cytochenlical Localization of Cell Products in Plant Tissue Culture.- 1 Introduction.- 2 The Laticifer.- 3 The Tissue Culture System for the Asclepiadaceae.- 4 Future Uses of Immunological-Cytochemical Techniques in Tissue Culture.- References.- Measurement of Oat Globulin by Radioimmunoassay.- 1 Introduction.- 2 Requirements for the Radioimmunoassay.- 3 Designing the RIA.- 4 Summary.- References.- Immunocytocheniistry of Chloroplast Antigens.- 1 Introduction.- 2 Light Microscopy.- 3 Electron Microscopy.- References.