NMR of Biological Macromolecules -

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Résumé :
Provided here are the latest techniques of NMR as applied to the study of proteins, carbohydrates and nucleic acids. The first chapters are devoted to an introduction to NMR and parameters related to molecular structure and molecular interactions. NMR experiments from basic 1D to 2D, 3D and 4D, used in combination with isotopically labelled molecules, are described and a general strategy is presented for biomacromolecular structure determination. Subsequent chapters deal with more advanced principles and techniques and their applications to structural and dynamic processes involving biomacromolecules in solution. Advanced results on peptide, protein, oligosaccharide and nucleic acid structure and recognition are presented.

Sommaire:
Basic Pulsed NMR Experiments.- Principles of Multidimensional NMR.- Understanding Pulsed FTNMR Experiments.- Computer - Assisted Structure Determination of Biomacromolecules by NMR.- Nuclear Shielding and Biomolecular Structure.- NMR Studies of Protein Ligand Interactions.- NMR Studies of Ligand - Protein Interactions Involving Quadrupolar Nuclei.- Multidimensional NMR Spectroscopy of Peptides.- Methods Used in the Assignments of the 1H and 15N Resonances of the c-Abl src Homology 2(SH2) Domain.- NMR and Unpaired Electrons in Biomolecules.- Long Time Protein Dynamics.- Local Dynamics of Macromolecules.- Structural and Conformational Studies of Glycoproteins and Oligosaccharide Recognition Determinants.- 13C Nuclear Magnetic Relaxation of Carbohydrate Molecules in Solution.- Dynamic Modelling of Polysaccharide Molecules in Solution..- 13C - lH Spin - Coupling Constants in Furanosyl Rings: Application to Oligonucleotide Structure Determination.- Through Bond Sugar - Phosphate Backbone Assignment in 13C labeled RNA by Triple Resonance 1H 13C and 31P NMR Spectroscopy.- NMR Studies of Protein - Nucleic Acid Interaction: The LAC Operon.- Determination of High - Resolution, Sequence - Dependent DNA Duplex Structures in Solution.- List of participants.