Natural Killer Cell Protocols -

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Résumé :
As the research has continued, it has become increasingly clear that natural killer (NK) cells are critical sentinels of the innate immune response, playing important roles in protecting the body from numerous pathogens and cancer in addition to contributing to normal pregnancy and impacting the outcomes of transplantation. While the first edition provided a valuable collection of classical cellular and in vivo techniques to study NK cell functions, the Second Edition of Natural Killer Cell Protocols: Cellular and Molecular Methods brings together more recently developed methods, more refined techniques, and detailed protocols designed to study NK cells within specialized tissue sites in both mice and humans. In this collection of methods, international leaders in the field cover topics ranging from the analysis of the various stages of NK cell development and maturation to specialized techniques for the identification of ligands for NK cell receptors. This volume also includes an appendix, providing a rich resource summarizing available reagents to study NK cells, cross-referencing KIR nomenclature, and detailing the many HLA ligands for various KIR family members. As a volume in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and thorough notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Natural Killer Cell Protocols: Cellular and Molecular Methods, Second Edition seeks to aid researchers and further advance our understanding of the functions, maturation, and regulation of these fascinating and dynamic cells.

Sommaire:
1. Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils Aharon G. Freud and Michael A. Caligiuri 2. In Vitro Development of Human Killer-Immunoglobulin Receptor Positive NK Cells Frank Cichocki and Jeffrey S. Miller 3. Subset Analysis of Human and Mouse Mature NK Cells Yoshihiro Hayakawa, Daniel M. Andrews, and Mark J. Smyth 4. Assessing Licensing of NK Cells A. Helena Jonsson and Wayne M. Yokoyama 5. Use of Stem Cell Radiation Chimeras to Analyze How Domains of Specific Proteins Impact on Murine NK Cell Development in vivo Rebecca H. Lian and Vinay Kumar 6. Use of Transfected Drosophila S2 Cells to Study NK Cell Activation Michael E. March, Catharina C. Gross, and Eric O. Long 7. Natural Killer Cell Conjugate Assay Using Two-Color Flow Cytometry Deborah N. Burshtyn and Chelsea Davidson 8. Studying NK Cell / Dendritic Cell Interactions Mathias Lucas, Cedric Vonarbourg, Peter Aichele, and Andreas Diefenbach 9. Analysis of the NK Cell Immunological Synapse Keri B. Sanborn, Gregory Rak, Ashley Mentlik, Pinaki P. Banerjee, and Jordan S. Orange 10. Measuring Intracellular Calcium Signaling in Murine NK Cells by Flow Cytometry Alexander W. MacFarlane IV, James F. Oesterling, and Kerry S. Campbell 11. Intracellular Staining for Analysis of the Expression and Phosphorylation of Signal Transducers and Activators of Transcription (STATs) in NK Cells Takuya Miyagi, Seung-Hwan Lee, and Christine A. Biron 12. A Model System for Studying NK Cell Receptor Signaling Lukasz K. Chlewicki and Vinay Kumar 13. Expression of cDNAs in Human Natural Killer Cell Lines by Retroviral Transduction S. M. Shahjahan Miah and Kerry S. Campbell 14. Lentiviral Gene Transduction inHuman and Mouse NK Cell Lines Ram Savan, Tim Chan, and Howard A. Young 15. Introduction of shRNAs into Human NK-like Cell Lines with Retrovirus Amanda K. Purdy and Kerry S. Campbell 16. Introduction of shRNAs into Primary NK Cells with Lentivirus Sam K.P. Kung 17. Methods to Identify and Characterize Different NK Cell Receptors and Their Ligands Dikla Lankry, Roi Gazit, and Ofer Mandelboim 18. Generating NK Cell Receptor-Fc Chimera Proteins from 293T Cells and Considerations of Appropriate Glycosylation Alon Zilka, Michal Mendelson, Benyamin Rosental, Oren Hershkovitz, and Angel Porgador 19. Identification of NK Cell Receptor Ligands Using a Signaling Reporter System Yoshie-Matsubayashi Iizuka, Nikunj V. Somia, and Koho Iizuka 20. Determining Ligand Specificity of Ly49 Receptors Kerry J. Lavender and Kevin P. Kane 21. Probing the Interactions of NK Cell Receptors with Ligand Expressed in trans and cis. Jonathan Back, L?onardo Scarpellino, and Werner Held 22. A Simple Method to Measure NK Cell Cytotoxicity in vivo Aurore Saudemont, Shannon Burke, and Francesco Colucci 23. Functional Analysis of Human NK Cells by Flow Cytometry Yenan T. Bryceson, Cyril Fauriat, Jo?o M. Nunes, Stephanie M. Wood, Niklas K. Bj?rkstr?m, Eric O. Long, and Hans-Gustaf Ljunggren 24. Analysis of the KIR Repertoire in Human NK Cells by Flow Cytometry Niklas K. Bj?rkstr?m, Cyril Fauriat, Yenan T. Bryceson, Johan K. Sandberg, Hans-Gustaf Ljunggren, and Karl-Johan Malmberg 25. KIR Genotyping by Multiplex PCR-SSP Smita Kulkarni, Maureen P. Martin, and Mary Carrington 26. Identification and Analysis of Novel Transcripts and Promoters in the Human Killer Cell Immunoglobulin-like Receptor (KIR) Genes Hongchuan Li, Paul W.