Phospho-Proteomics -

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Résumé :
This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phosphoproteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Phosphoproteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Sommaire:
Part I: Techniques for Tagging Phospho-Moieties?1. Thiol-ene Enabled Detection of Thiophosphorylation as a Labeling Strategy for PhosphoproteinsKaelyn E. Wilke and Erin E. Carlson?2. Phosphopeptide Detection with Biotin-Labeled Phos-tagEmiko Kinoshita-Kikuta, Eiji Kinoshita and Tohru Koike?3. Phosphopeptide Enrichment by Covalent Chromatography after Solid Phase Derivatization of Protein Digests on Reversed Phase Supports Heinz Nika, Ruth Hogue Angeletti, and David H. Hawke?Part II: Labeling Techniques for Quantitative Phosphoproteomics?4. Peptide Labeling Using Isobaric Tagging Reagents for Quantitative PhosphoproteomicsLei Cheng, Trairak Pisitkun, Mark A. Knepper, and Jason D. Hoffert?5. Identification of Direct Kinase Substrates Using Analogue-Sensitive AllelesDaniel A. Rothenberg, Elizabeth A. Gordon, Forest M. White, and Sebastian Lourido?6. Quantitative Analysis of Tissue Samples by Combining iTRAQ Isobaric Labeling with Selected/Multiple Reaction Monitoring (SRM/MRM)Ryohei Narumi ?and Takeshi Tomonaga??Part III: Phosphopeptide Enrichment Strategies ?7. Enrichment Strategies in PhosphoproteomicsAlexander Leitner?8. Phosphopeptide Enrichment by Immobilized Metal Affinity ChromatographyTine E. Thingholm and Martin R. Larsen?9. The use of Titanium Dioxide for Selective Enrichment of Phosphorylated PeptidesTine E. Thingholm and Martin R. Larsen?10. Sequential Elution from IMAC (SIMAC) ? An Efficient Method for Enrichment and Separation of Mono- and Multi-Phosphorylated PeptidesTine E. Thingholm and Martin R. Larsen?11. Improving the Phosphoproteome Coverage for Limited Sample Amounts Using TiO2-SIMAC-HILIC (TiSH) Phosphopeptide Enrichment and FractionationKasper Engholm-Keller and ?Martin R. Larsen?12. Offline High pH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome CoverageTanveer S Batth, Jesper V Olsen?13. Phosphopeptide Enrichment Using Various Magnetic Nanocomposites ? An Overview?ris L. Batalha and Ana Cec?lia A. Roque??Part IV: Phosphoprotein Analysis by Mass Spectrometry?14. Two Dimensional Gel Electrophoresis-Based Plant Phosphoproteomics?Chao Han and Pingfang Yang?15. Variable Digestion Strategies for Phosphoproteomics AnalysisHumberto Gonczarowska-Jorge, Margherita Dell?Aica, Clarissa Dickhut, and Ren? P. Zahedi?16. On-line LC-FAIMS-MS/MS for the Analysis of Phosphorylation in ProteinsHongyan Zhao, Andrew J. Creese, and Helen J. Cooper?17. Simple and Reproducible Sample Preparation for Single Shot Phosphoproteomics With High SensitivityRosa R. Jersie-Christensen, Abida Sultan, and Jesper V. Olsen??Part V: High-throughput Studies of the Phosphoproteome ?18. Identification of Direct Kinase Substrates via Kinase Assay Linked PhosphoproteomicsLiang Xue, Justine V. Arrington, and W. Andy Tao?19. Phosphoprotein Detection by High-Throughput Flow CytometryJohannes Landskron and Kjetil Task?n??Part VI: Bioinformatics Resources for Phosphoproteomics Analyses?20. Resources for Assignment of Phosphorylation Sites on Peptides and Proteins Vaishnavi Ravikumar, Boris Macek, and Ivan Mijakovic?21. From Phosphosites to KinasesStephanie Munk, Jan C. Refsgaard, and Jesper V. Olsen, and Lars J. Jensen?22. Search Databases and Statistics ? Pitfalls and Best Practices in PhosphoproteomicsJan C. Refsgaard, Stephanie Munk, and Lars J. Jensen?23. Systems Analysis for Interpretation of Phosphoproteomics DataStephanie Munk, Jan C. Refsgaard, and Jesper V. Olsen?