Functional Genomics -

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Résumé :

Prediction of Protein Tertiary Structures using MUFOLD.-Prediction of Protein Functions.-Genome Wide Screens for Expressed Hypothetical Proteins.-Self Custom-Made SFP Arrays for Non-Model Organisms.-Construction and Analysis of Full-length and Normalized cDNA Libraries from Citrus.-Assembling Linear DNA Templates for in vitro Transcription and Translation.-Automated Computational Analysis of Genome-wide DNA Methylation Profiling Data??from HELP-tagging Assays.-Detection of RNA Editing Events in Human Cells using High- Throughput? ??Sequencing.-Comparative Study of Differential Gene Expression in Closely Related Bacterial?Species by Comparative Hybridization.-Whole Genome RT-qPCR MicroRNA Expression Profiling.-Using Quantitative Real-Time Reverse Transcriptase Polymerase Chain Reaction to?Validate Gene Regulation by PTTG.-FRET-BasedReal-Time DNA Microarrays.-?2-D Gel Electrophoresis: Constructing 2D-Gel Proteome Reference Maps.-The use of Antigen Microarrays in Antibody Profiling.-Limited Proteolysis in Proteomics using Protease-immobilized Microreactors.-Mass Spectrometry for Protein Quantification in Biomarker Discovery.-High-throughput Microtitre Plate-based Assay for DNA Topoisomerases.-Microscale Thermophoresis as a Sensitive Method to Quantify Protein - Nucleic?Acid Interactions in Solution.-Bioluminescence Resonance Energy Transfer: an Emerging Tool for the Detection of?Protein-protein Interaction in Living Cells.-LuMPIS - Luciferase-based MBP-pull-down Protein Interaction Screening System.-Yeast Two-Hybrid Screens: Improvement of Array-based Screening Results by N- and??C-terminally Tagged Fusion Proteins.-Inducible microRNA-mediated Knockdown of the Endogenous Human Lamin A/C Gene.-?Multiple-gene Silencing using Antisense RNAs in Escherichia coli.-Functional Screen of Zebrafish Deubiquitylating Enzymes by Morpholino Knockdown?and insitu Hybridization.-Silencing of Gene Expression by GymnoticDelivery of Antisense Oligonucleotides.-Polycistronic Expression of Interfering RNAs from RNA Polymerase III Promoters.-Metabolite Analysis of Cannabis sativaL. by NMR Spectroscopy.-Metabolome Analysis of Gram-positive Bacteria such as Staphylococcus??? aureus by GC-MS and LC-MS.-Metabolic Fingerprinting Using Comprehensive Two-dimensional Gas ??Chromatography - Time-of-flight Mass Spectrometry (GC?GC-TOF-MS).

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