Stem Cell Migration -

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Résumé :
The migration of stem cells has been found to be critical during early development for the organization of the embryonic body as well as during adult life with tissue homeostasis and regeneration of organ function.? Due to the low frequency of these cells in vivo,? problems in identifying and prospectively purifying tissue specific stem cells near homogeneity, and, most importantly, a lack of adequate technologies and protocols to study stem cell migration in vivo, this vital research has been quite difficult until recently.? In Stem Cell Migration: Methods and Protocols, experts in the field compile and highlight the standard and novel techniques that allow the studying of the migration of stem cells in one succinct manual. Including protocols on germ, neuronal, and hematopoietic stem cells, during development and adulthood with a clear emphasis on in vivo technologies, the volume also extends its coverage to in vitro approaches toward several developmentally-conserved signaling pathways.? Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ? Practical and convenient, Stem Cell Migration: Methods and Protocols provides key, state-of the art information on experimental techniques for studying stem cell migration both at a cellular and molecular level in development, regeneration, and disease.

Sommaire:
Part I: Stem Cells and Migration: An Overview?1. Trafficking of Stem Cells??????????? Claire Magnon, Daniel Lucas, and Paul S. Frenette?2. Migratory Strategies of Normal and Malignant Stem Cells??????????? David A. Hess and Alison L. Allan?Part II: Stem Cells Identification and Microscopic Technologies to Track Transplanted Stem Cells In Vivo?3. Hematopoietic Stem Cell Characterization and Isolation??????????? Lara Rossi, Grant A. Challen, Olga Sirin, Karen Kuan-Yin Lin, and Margaret A. Goodell?4. Isolation and Characterization of Adult Neural Stem Cells??????????? Florian A. Siebzehnrubl, Vinata Vedam-Mai, Hassan Azari, Brent A. Reynolds, and Loic P. Deleyrolle?5. Magnetic Resonance Imaging of Stem Cell Migration??????????? Eva Sykov? Pavla Jendelov?, and V?t Herynek?6. Imaging of Schwann Cells In Vivo??????????? Rahul Kasukurthi and Terrence M. Myckatyn?7. Imaging of Embryonic Stem Cell Migration In Vivo??????????? Andrew S. Lee and Joseph C. Wu?Part III: Stem Cell Migration During Development?8. In Vivo Germ Line Stem Cell Migration:? A Mouse Model??????????? Brian Dudley and Kathleen Molyneaux?9. Live Microscopy of Neural Stem Cell Migration in Brain Slices??????????? Jin-Wu Tsai and Richard B. Vallee?10. Whole Embryo Imaging of Hematopoietic Cell Emergence and Migration??????????? Michael J. Ferkowicz and Mervin C. Yoder?11. Stem Cell Migration: A Zebrafish Model??????????? Pulin Li and Leonard I. Zon?12. Imaging Pluripotent Cell Migration in Drosophila??????????? Michael J. Murray and Robert Saint?Part IV: Adult Hematopoietic Stem Cell Migration?13. Adhesion, Migration, and Homing of Murine Hematopoietic Stem Cells and Progenitors??????????? Jose A. Cancelas?14. Methods to Analyze the Homing Efficiency and Spatial Distribution of Hematopoietic Stem and Progenitor Cells and Their Relationship to the Bone Marrow Endosteum and Vascular Endothelium??????????? Jochen Grassinger and Susan K. Nilsson?15. Imaging Hematopoietic Stem Cells in the Marrow of Long Bones In Vivo??????????? Anja K?hler, Hartmut Geiger, and Matthias Gunzer?Part V: Non Hematopoietic Stem Cell Migration?16. Studies of Adult Neural Stem Cell Migration??????????? Adam C. Puche and Serena Bovetti?17. Dissecting Mesenchymal Stem Cell Movement: Migration Assays for Tracing and Deducing Cell Migration??????????? Erika L. Spaeth and Frank Marini?18. Epithelial Stem Cells??????????? Kyle M. Draheim and Stephen Lyle?Part VI: Pathways Implicated in Stem Cell Migration?19. Pathways Implicated in Stem Cell Migration:? The SDF-1/CXCR4 Axis??????????? Yaron Vagima, Kfir Lapid, Orit Kollet, Polina Goichberg, Ronen Alon, and Tsvee Lapidot?20. The Role of Receptor Tyrosine Kinases in Primordial Germ Cell Migration??????????? Louise Silver-Morse and Willis X. Li?21. Rho GTPases in Hematopoietic Stem/Progenitor Cell Migration??????????? Wei Liu, Yuxin Feng, Xun Shang, and Yi Zheng