Malaria -

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Résumé :

In vitro Culturing P. falciparum Erythrocytic Stages.-Production of Plasmodium falciparumGametocytes in vitro.-In vitro Differentiation of Plasmodium falciparum Gametocytes into Ookinetes.-Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro Culture of Plasmodium Knowlesi Blood Stages.-Laboratory Maintenance of Rodent Malaria Parasites.-Transfection of Plasmodium falciparum.-Transfection of Rodent Malaria Parasites.-Recombination-mediated Genetic Engineering of Plasmodium berghei DNA.-Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database.-Transient Transfection of Plasmodium vivax Blood-stage Parasites.-Silencing of Genes and Alleles by RNAi in Anopheles gambiae.-Analysis of Variant Gene Family Expression by Quantitative PCR.-DNA Microarray-based Genome-wide Analyses of Plasmodium Parasites.-Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray Platform.-Transcriptome Analysis using RNA-seq.-Experimental Tools for the Study of Protein Phosphorylation in Plasmodium.-Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic Analysis.-Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparum.-Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparumMerozoites.-Microsphiltration: a Microsphere Matrix to Explore Erythrocyte Deformability.-Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition Events.-Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System.-Genome-wide Chromatin Immunoprecipitation-sequencing in Plasmodium.-In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparum.-Bioluminescence Imaging of P. bergheiSchizont Sequestration in Rodents.-Scoring Sporozoite Motility.-Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry.-Imaging Sporozoite Cell Traversal in the Liver of Mice.-Analysis of Liver Stage Development in, and Merozoite Release from Hepatocytes.-Quantitative Analysis of Plasmodium bergheiLiver Stages by Bioluminescence Imaging.-Activation of Human NK Cells by Plasmodium-infected Red Blood Cells.-Chimeric Parasites as Tools to Study Plasmodium?Immunology and Assess Malaria Vaccines.-Development and Use of TCR Transgenic Mice for Malaria Immunology Research.-Tracking the Total CD8 T Cell Response Following Whole PlasmodiumVaccination .-Screening Inhibitors of P. bergheiBlood Stages using Bioluminescent Reporter Parasites.-Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using Cytometry.-Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug Cure.-Vaccination using Radiation-or Genetically-Attenuated Live Sporozoites.-Vaccination using Normal Live Sporozoites Under Drug Treatment.-Assessing Transmission Blockade in Plasmodium spp. .-Mosquito Transgenic Technologies to Reduce Plasmodium Transmission..-Silencing of Genes and Alleles by RNAi in Anopheles gambiae.-Analysis of Variant Gene Family Expression by Quantitative PCR.-DNA Microarray-based Genome-wide Analyses of Plasmodium Parasites.-Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray ...

Biographie:
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Sommaire:

In vitro Culturing P. falciparum Erythrocytic Stages.-Production of Plasmodium falciparum Gametocytes in vitro .- In vitro Differentiation of Gametocytes into Ookinetes.- Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi Culture of Plasmodium Knowlesi Blood Stages.-Laboratory Maintenance of Rodent Malaria Parasites.-Transfection of .-Transfection of Rodent Malaria Parasites.-Recombination-mediated Genetic Engineering of Plasmodium berghei DNA.-Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database.-Transient Transfection of Plasmodium vivax Blood-stage Parasites.-Silencing of Genes and Alleles by RNAi in Anopheles gambiae .-Analysis of Variant Gene Family Expression by Quantitative PCR.-DNA Microarray-based Genome-wide Analyses of Plasmodium Parasites.-Whole-genome Analysis of spp . Utilizing a New Agilent Technologies DNA Microarray Platform.-Transcriptome Analysis using RNA-seq.-Experimental Tools for the Study of Protein Phosphorylation in .-Extraction of Hydrophilic Metabolites from -infected Erythrocytes for Metabolomic Analysis.-Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in .-Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Merozoites.-Microsphiltration: a Microsphere Matrix to Explore Erythrocyte Deformability.-Atomic Force Microscopy of -infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition Events.-Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System.-Genome-wide Chromatin Immunoprecipitation-sequencing in In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of .-Bioluminescence Imaging of P. berghei Schizont Sequestration in Rodents.-Scoring Sporozoite Motility.-Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry.-Imaging Sporozoite Cell Traversal in the Liver of Mice.-Analysis of Liver Stage Development in, and Merozoite Release from Hepatocytes.-Quantitative Analysis of Liver Stages by Bioluminescence Imaging.-Activation of Human NK Cells by -infected Red Blood Cells.-Chimeric Parasites as Tools to Study Plasmodium? Immunology and Assess Malaria Vaccines.-Development and Use of TCR Transgenic Mice for Malaria Immunology Research.-Tracking the Total CD8 T Cell Response Following Whole Vaccination .-Screening Inhibitors of Blood Stages using Bioluminescent Reporter Parasites.-Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using Cytometry.-Induction of anti- Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug Cure.-Vaccination using Radiation-or Genetically-Attenuated Live Sporozoites.-Vaccination using Normal Live Sporozoites Under Drug Treatment.-Assessing Transmission Blockade in spp. .-Mosquito Transgenic Technologies to Reduce Transmission..-Silencing of Genes and Alleles by RNAi in Plasmodiumfalciparum Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug Cure.-Vaccination using Radiation-or Genetically-Attenuated LiveSporozoites.-Vaccination using Normal Live Sporozoites Under Drug Treatment.-Assessing Transmission Blockade in Transmission.