In Vitro Neurotoxicology -

. .

Résumé :

In Vitro Neurotoxicology: An Introduction.- Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies.- Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures.- Microglia Cell Culture: A Primer for the Novice.- Neural Stem Cells for Developmental Neurotoxicity Studies.- Preparation, Maintenance, and Use of Serum-Free Aggregating Brain Cell Cultures.- Neurospheres as a Model for Developmental Neurotoxicity Testing.- Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures.- Glia-Neuron Sandwich Co-Cultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity.- In Vitro Models to Study the Blood Brain Barrier.- Measurements of Cell Death in Neuronal and Glial Cells.- Measurements of Neuronal Apoptosis.- Measurement of Isoprostanes as Markers of Oxidative Stress.- Assessment of Glutathione Homeostasis.- Assessing Neuronal Bioenergetic Status.- Fluorescent Assessment of Intracellular Calcium in Individual Cells.- Homeostatic Regulation of Glutamate Neurotransmission in Primary Neuronal Cultures.- Detection of Nitric Oxide Formation in Primary Neural Cells and Tissues.- JNK3-Mediated Apoptotic Cell Death in Primary Dopaminergic Neurons.- Measurement of Proteasomal Dysfunction in Cell Models of Dopaminergic Degeneration.- Cell Signaling and Neurotoxicity: Protein Kinase C In Vitro and In Vivo.- Cell Signaling and Neurotoxicity: ³H-Arachidonic Acid Release (Phospholipase A₂) in Cerebellar Granule Neurons.- Quantitative Assessment of Neurite Outgrowth in PC12 Cells.- Measurements of Astrocyte Proliferation.- Quantification of Synaptic Structure Formation in Co-Cultures ofAstrocytes and Hippocampal Neurons.- Volume Measurements in Cultured Primary Astrocytes.- Assessment of Cholesterol Homeostasis in Astrocytes and Neurons.- Dose-Response or Dose-Effect Curves in In Vitro Experiments and Their Use to Study Combined Effects of Neurotoxicants.