Mammalian Chromosome Engineering -

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Résumé :
The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry.? Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system.? Written in the highly successful Methods in Molecular Biology? series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ?Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.

Sommaire:
1. Developing Extrachromosomal Gene Expression Vector Technologies: An Overview??????????? Richard Wade-Martins?2. High Capacity Extrachromosomal Gene Expression Vectors??????????? Olivia Hibbitt and Richard Wade-Martins?3. Naturally Occurring Minichromosome Platforms in Chromosome Engineering: An Overview??????????? Elena Raimondi?4. Chromosome Transfer Via Cell Fusion??????????? Marianna Paulis?5. Insertional Engineering of Chromosomes with Sleeping Beauty Transposition: An Overview??????????? Ivana Grabundzija, Zsuzsanna Izsv?k, and Zolt?n Ivics?6. Rodent Transgenesis Mediated by a Novel Hyperactive Sleeping Beauty Transposon System??????????? Lajos M?t?s?7. Construction and Use of a Bottom-Up HAC Vector for Transgene Expression??????????? Masashi Ikeno and Nobutaka Suzuki?8. De novo Generation of Satellite DNA-Based Artificial Chromosomes by Induced Large-Scale Amplification??????????? Erika Csonka?9. Downstream Bioengineering of ACE Chromosomes for Incorporation of Site-Specific Recombination Cassettes??????????? Amy L. Greene and Edward L. Perkins?10. Chromosome Engineering with Lambda-Integrase Mediated Recombination System: The ACE System??????????? T?nde Praznovszky?11. Dendrimer Mediated Transfer of Engineered Chromosomes??????????? Robert L. Katona?12. Engineered Chromosomes in Transgenics??????????? Peter Blazso, Ildiko Sinko, and Robert L. Katona?13. Transfer of Stem Cells Carrying Engineered Chromosomes with XY Clone Laser System??????????? Ildiko Sinko and Robert L. Katona?14. Mammalian Artificial Chromosomes and Clinical Applications for Genetic Modification of Stem Cells: An Overview??????????? Robert L. Katona, Sandra L. Vanderbyl, and Carl F.Perez?15. Engineered Mammalian Chromosomes in Cellular Protein Production: Future Prospects??????????? Malcolm L. Kennard