Influenza Virus -

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Résumé :
Reports of influenza-like illnesses date back to the Middle Ages, and outbreaks of influenza likely afflicted humans long before that. Over the last half century, influenza virus research has led to the development of two classes of antivirals ? ion channel and neuraminidase inhibitors. Recently, a method of the artificial generation of an influenza virus was established. This system has been instrumental in the development of novel influenza vaccines and in the understanding of viral pathogenicity and the functions of viral proteins. Influenza Virus: Methods and Protocols summarizes the current techniques that have made this progress possible, ranging from protocols for virus isolation, growth, and subtyping to procedures for the efficient generation of any influenza virus. Written in the successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. ? Authoritative and easily accessible, Influenza Virus: Methods and Protocols seeks to serve both professionals and novices with the techniques used in numerous laboratories around the world that are, thus, the building blocks that underpin almost all influenza virus research.

Biographie:
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Sommaire:

1. Influenza Viruses - An Introduction

??????????????? Yoshihiro Kawaoka and Gabriele Neumann

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2. Influenza Virus Isolation

??????????????? Scott Krauss, David Walker, and Robert G. Webster

3. Influenza Virus Titration, Antigenic Characterization, and Serological Methods for Antibody Detection

??????????????? Alexander Klimov,? Amanda Balish, Vic Veguilla, Hong Sun, Jarad Schiffer, Xiuhua Lu, Jacqueline M. Katz, and Kathy Hancock

4. Diagnosis of Influenza Virus

??????????????? Kirsten St. George

5. Solid-phase Assays of Receptor-binding Specificity

??????????????? Mikhail N. Matrosovich and Alexandra S. Gambaryan

6. The Chemiluminescent Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors

??????????????? Margaret Okomo-Adhiambo, Aeron C. Hurt, and Larisa V. Gubareva

7. The Fluorescence Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors

??????????????? Aeron C. Hurt, Margaret Okomo-Adhiambo, and Larisa V. Gubareva

8. Animal Models

??????????????? Edwin J.B. Veldhuis Kroeze, Thijs Kuiken, and Albert D.M.E. Osterhaus

9. Influenza Virus Surveillance, Vaccine Strain Selection and Manufacture

??????????????? Klaus St?hr, Doris Bucher, Tony Colgate, and John Wood

10. Genetic Engineering of Live Attenuated Influenza Viruses

??????????????? Hong Jin, Zhongying Chen, Jonathan Liu, and George Kemble

11. Influenza A Virus Molecular Virology Techniques

??????????????? Bin Zhou and David E. Wentworth

12. Reverse Genetics of Influenza Viruses

??????????????? Gabriele Neumann, Makoto Ozawa, and Yoshihiro Kawaoka

13. Genetic Analysis

??????????????? Gavin J.D. Smith, Justin Bahl, and Vijaykrishna Dhanasekaran