Microchip Capillary Electrophoresis Protocols -

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Résumé :
In this volume expert researchers in the field detail the operations of microchip capillary electrophoresis. Chapters focus on small molecule, biomolecule applications, various detection modes, and sample preparation approaches are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microchip Capillary Electrophoresis Protocol aids scientists in continuing to study microchip capillary electrophoresis.

Sommaire:
Part I. Overview?1. An Overview of the use of Microchips in Electrophoretic Separation Techniques: Fabrication, Separation Modes, Sample Preparation Opportunities, and On-Chip DetectionStijn Hendrickx, Wim De Malsche, and Deirdre Cabooter?Part II. Microchip Capillary Electrophoresis Of Small Molecules2. Microchip Electrophoresis for Fast and Interference-Free Determination of Trace Amounts of Glyphosate and Glufosinate Residues in Agricultural ProductsXuan Wei And Qiaosheng Pu?3. Microchip Capillary Electrophoresis Of Nitrite and Nitrate in Cerebrospinal FluidMari?n Mas?r, R?bert Bodor, and Peter Tro?ka?4. Analysis Of Thiols By Microchip Capillary Electrophoresis for In Situ Planetary InvestigationsMaria F. Mora, Amanda M. Stockton, and Peter A. Willis?5. Determination of Ofloxacin in Ofloxacin Ear Drops by Microfluidic Chip with Contactless Conductivity DetectionBing Chen and Kaicheng Li??Part III. Microchip Capillary Electrophoresis Of Nucleic Acids, Proteins, Peptides, Pathogens?6. Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria ImmunoseparationSona Krizkova, Hoai Viet Nguyen, Maja Stanisavljevic, Pavel Kopel, Marketa Vaculovicova, Vojtech Adam, and Rene Kizek?7. Fast High-Throughput Screening Of H1N1 Virus by Parallel Detection with Multi-Channel Microchip ElectrophoresisPeng Zhang, He Nan, Seungah Lee, and Seong Ho Kang?8. Sex Identification of Ancient DNA Samples using a Microfluidic DeviceKirsty J Shaw, Keri A. Brown, Terence A. Brown, and Stephen J. Haswell??9. Fast and Continuous-Flow Detection and Separation Of DNA-Complexes and DNA in Nanofluidic Chip FormatMartina Viefhues, Jan Regtmeier, and Dario Anselmetti ?10. Multi-Dimension Microchip-Capillary Electrophoresis Device for Determination of Functional Proteins in Infant Milk FormulaRuige Wu, Zhiping Wang, and Ying Sing Fung?11. Fast Assembly of Anti-Voltage Photonic Crystals in Microfluidic Channels for Ultrafast Separation of Amino Acids and PeptidesYi Chen, Tao Liao and Can Hu?Part IV. Nonaqueous Applications12. Rapid Determination of Catecholamines in Urine Samples by Nonaqueous Microchip Electrophoresis with LIF DetectionHongmei Hu and Yuanming Guo?Part V. Capillary Electrochromatography On Microchips13. Carbon Nanotube Based Separation Columns for Microchip ElectrochromatographyK. B. Mogensen, B. Delacourt, and J. P. Kutter?14. Electrochromatography on Acrylate-Based Monolith in Cyclic Olefin Copolymer Microchip: an Attractive? TechnologyY. Ladner, G. Cretier, and K. Faure?Part VI. Sample Preparation Approaches15. On-Chip Electromembrane Extraction for Monitoring Drug Metabolism in Real Time by Electrospray Ionization Mass SpectrometryNickolaj J. Petersen, Henrik Jensen, and Stig Pedersen-Bjergaard?16. Sample Preparation for N-Glycosylation Analysis of Therapeutic Monoclonal Antibodies By Electrophoresis?kos Szekr?nyes, Jan Partyka, Csaba Varadi, Jana Krenkova, Frantisek Foret, and Andr?s Guttman?