Notch Signaling -

. .

Résumé :
Notch Signaling: Methods and Protocols focuses on molecular, biochemical and bioinformatics aspects of Notch signaling. Chapters analyze the Notch interactome, post-translational modifications of Notch, ligand binding assays, methods to assess proteolytic cleavage and transcriptional targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Notch Signaling: Methods and Protocols is a valuable contribution to hundreds of labs and thousands of scientists who pursue this research area with vigor.

Sommaire:
1. Introduction to Notch SignalingShinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen?2. Genetic Screens to Identify New Notch Pathway Mutants in DrosophilaNikolaos Giagtzoglou3. Structure-function Analysis of Drosophila Notch using Genomic Rescue TransgenesJessica Leonardi and Hamed Jafar-Nejad?4. Overview of Genetic Tools and Techniques to Study Notch Signaling in MiceThomas Gridley and Andrew K. Groves ?5. Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogasterEmiliana Tognon and Thomas Vaccari?6. Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in DrosophilaLydie Couturier and Fran?ois Schweisguth?7. Tracking Trafficking of Notch and its Ligands in Mammalian CellsPatricia Chastagner and Christel Brou?8. Visualizing Notch Signaling in vivo in Drosophila TissuesBenjamin E. Housden, Jinghua Li, and Sarah J. Bray ?9. Monitoring Notch Activity in the MouseLavinia Alberi and Swananda Marathe?10. Notch Signaling Assays in Drosophila Cultured Cell LinesJinghua Li, Benjamin E. Housden, and Sarah J. Bray?11. Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter AssaysMa. Xenia G. Ilagan and Raphael Kopan?12. Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging AssaysMa. Xenia G. Ilagan and Raphael Kopan?13. Visualization of Notch Signaling Oscillation in Cells and TissuesHiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama?14. Proteomic Analysis of the Notch InteractomeK. G. Guruharsha, Kazuya Hori, Robert A. Obar, and Spyros Artavanis-Tsakonas?15. Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its LigandsPat Whiteman, Christina Redfield and Penny A. Handford?16. Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry?Shinako Kakuda and Robert S. Haltiwanger?17. Assay to Probe Proteolytic Processing of Notch by ?-secretaseLutgarde Serneels, Ina Tesseur and Bart De Strooper ?18. Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift AssayKelly L. Arnett and Stephen C. Blacklow?19. Identifying Direct Notch Transcriptional Targets using the GSI-washout AssayWill Bailis, Yumi Yashiro-Ohtani and Warren S. Pear?20. Probing the Epigenetic Status at Notch Target GenesTilman Borggrefe and Robert Liefke?21. Notch-ligand Binding Assays in Drosophila CellsAiguo Xu and Kenneth D. Irvine?22. Modeling Notch Signaling: A Practical TutorialPau Formosa-Jordan and David Sprinzak??23. Small Molecules that Inhibit Notch SignalingGerdien E. De Kloe and Bart De Strooper24. Application and Evaluation of Anti-Notch Antibodies to Modulate Notch SignalingWendy R. Gordon and Jon C. Aster?25. Application of Anti-ligand Antibodies to Inhibit Notch SignalingJun-ichiro Koga and Masanori Aikawa?