Mouse Genetics -

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Résumé :

Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre-loxP Site-Specific Recombination.- Generation of Conditional Knockout Mice.- Generating Mouse Lines for Lineage Tracing and Knockout Studies.- Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE).- Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection.- Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases.- Intra cytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice.- Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice.- Generation of Genetically Engineered Mice by the piggyBac Transposon System.- Generation and Applications of MADM-Based Mouse Genetic Mosaic System.- Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System.- In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis.- Isolation and Handling of Mouse Embryonic Fibroblasts.- Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue.- Pdx1 (GFP/w) Mice for Isolation, Characterization and Differentiation of Pancreatic Progenitor Cells.- Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development.- Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System.- Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo.- Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts.- Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment.- Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty.- Generating Double Knockout Mice to Model Genetic Intervention for DiabeticCardiomyopathy in Humans.- Experimental Osteoarthritis Models in Mice.- Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI).- Mouse Models of Acute and Chronic Colitis.- Manipulation and Assessment of Gut Microbiome for Metabolic Studies.