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Présentation Argonaute Proteins de Format Broché
- Livre Sports
Résumé : Cloning and Identification of Recombinant-Argonaute Bound Small RNAs using Next-Generation Sequencing.-?Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins using SYBR Green-Based qRT-PCR.-?Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with?In Vitro?Loading Samples.-?Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant.-?MicroRNA Detection by Whole-Mount?In Situ?Hybridization in?C. elegans.-?cCLIP-Seq: Retrieval of Chimaeric Reads from HITS-CLIP (CLIP-Seq) Libraries.-?Kinetic Analysis of Small Silencing RNA Production by Human and?Drosophila?Dicer Enzymes?In Vitro.-?Nucleic Acid-Binding Assay of Argonaute Protein using Fluorescence Polarization.-?Reconstitution of RNA Interference Machinery.-?Single-Molecule Analysis for RISC Assembly and Target Cleavage.-?Profiling Open Chromatin Structure in the Ovarian Somatic Cells using ATAC-Seq.-?Assessing miR-451 Activity and Its Role in Erythropoiesis.-?Functional Analysis of MicroRNAs in Neurogenesis during Mouse Cortical Development.-?Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing.-?Genomic Tagging of?AG01?using CRISPR/Cas9-Mediated Homologous Recombination.-?Accurate Profiling and Quantification of tRNA Fragments from RNA-Seq Data: A?vade mecum?for MINTmap.
Biographie:
analysis of biochemical properties of Argonautes...
Sommaire:
This volume covers methods that analyze various Argonaute proteins from a variety of organisms to help researchers better understand their properties ranging from a molecular level to an organismal level. The chapters in this book explore the following topics: identification and expression analysis of guide nucleic acids and their targets...
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