Germline Stem Cells -

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Résumé :
This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter?s guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.

Sommaire:
Sarah L. Crittenden, Hannah S. Seidel, and Judith Kimble ? 2. ?Methods for Studying the Germline of the Human Parasite Schistosoma ?Mansoni Julie N.R. Collins and James J. Collins III 3.? Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells Mayu Inaba and Yukiko M. Yamashita 4. ?Live Imaging of the Drosophila Testis Stem Cell Niche Leah J. Greenspan and Erika L. Matunis 5. ?RNA Isolation from Early Drosophila Larval Ovaries Dana Gancz and Lilach Gilboa 6. ?Live-Cell Imaging of the Adult Drosophila Ovary using Confocal Microscopy Nevine A. Shalaby and Michael Buszczak ? 7. ?Measurement Of Mrna Poly(A) Tail Lengths In Drosophila Female Germ Cells and Germline Stem Cells Aymeric Chartier, Willy Joly, and Martine Simonelig 8.? Identification of Germline Stem Cells in Zebrafish Bruce W. Draper 9.? Primordial Germ Cell Isolation from Xenopus Laevis Embryos Amanda M. Butler, Tristan Aguero, Karen M. Newman, and Mary Lou King 10.? Single-Cell Lineage Analysis of Oogenesis in Mice Lei Lei and Allan C. Spradling 11. ?Visualization and Lineage Tracing Of Pax7+ Spermatogonial Stem Cells in the Mouse Gina M. Aloisio, Ileana Cuevas, Yuji Nakada, Christopher G. Pe?a, and Diego H. Castrillon 12. ?Transplantation as a Quantitative Assay to Study Mammalian Male Germline Stem Cells Aileen R. Helsel and Jon M. Oatley ? 13. ?Mouse Fetal Germ Cell Isolation and Culture Techniques Cassy M. Spiller, Guillaume Burnet, and Josephine Bowles 14. ?Rattus Norvegicus Spermatogenesis Colony Forming Assays F. Kent Hamra 15. ?Identification of Mouse Pirna Pathway Components Using Anti-MIWI2 Antibodies Takamasa Hirano, Hidetoshi Hasuwa, and Haruhiko Siomi