SH2 Domains -

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Résumé :

Introduction: History of SH2 Domains and Their Applications.-?What Have We Learned from SH2 Domains?.-?Hidden Markov Models for Protein Domain Homology Identification and Analysis.-?Classification and Lineage Tracing of SH2 Domains Throughout Eukaryotes.-?SH2 Ligand Prediction-Guidance for In-Silico Screening.-?An Efficient Semi-Supervised Learning Approach to Predict SH2 Domain Mediated Interactions.-?Proteomic Clustering Analysis of SH2 Domain Datasets.-?Expression and Production of SH2 Domain Proteins.-?Expression and Purification of Soluble STAT5b/STAT3 Proteins for SH2 Domain Binding Assay.-?Purification of SOCS (Suppressors of Cytokine Signalling) SH2 Domains for Structural and Functional Studies.-?Expression and Purification of SH2 Domains Using Baculovirus Expression System.-?Functionally Altered SH2 Domains for Biochemical Studies: Loss of Function Mutant and Domain Concatenation.-?Creation of Phosphotyrosine Superbinders by Directed Evolution of an SH2 Domain.-?Structural Characterization of Monomeric/Dimeric State of p59^fyn SH2 Domain.-?NMR Chemical Shift Mapping of SH2 Peptide Interactions.-?Calorimetric Measurement of SH2 Domain Ligand Affinities.-?Binding Assays Using Recombinant SH2 Domains: Far-Western, Pull Down, and Fluorescence Polarization.-?In-Solution SH2 Domain Binding Assay Based on Proximity Ligation.-?Alpha-Based Multiplexed Assay for Identifying SH2 Domain Antagonists.-?Characterizing SH2 Domain Specificity and Network Interactions Using SPOT Peptide Arrays.-?High Throughput Quantification of SH2 Domain-Phosphopeptide Interactions with Cellulose Peptide Conjugate Microarrays.-?SH2 Domains as Affinity Reagents for Phosphotyrosine Protein Enrichment and Proteomic Analysis.-?Identification of Tyrosine Phosphorylated Proteins by SH2 Domain Affinity Purification and Mass Spectrometry.-?Analysis of the Global Changes in SH2 Binding Properties Using Mass Spectrometry Supported by Quantitative Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technique.-?Using Reciprocal Protein-Peptide Array Screening to Unravel Protein Interaction Networks.-?Rosette Assay: Highly Customizable Dot-Blot for SH2 Domain Screening.-?Microwestern Arrays for Systems-Level Analysis of SH2 Domain-Containing Proteins.-?SH2 Binding Site Protection Assay: A Method for Identification of SH2 Domain Interaction Partners by Exploiting SH2 Mediated Phosphosite Protection.-?Real Time Single Molecule Visualization of SH2 Domain Membrane Recruitment in Growth Factor Stimulated Cells.-?SH2 Domain-Based FRET Biosensor for Measuring BCR-ABL Activity in Living CML Cells.-?SH2 Domain Histochemistry.