Microbial Steroids -

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Résumé :

Steroid Bioconversions.-?Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins.-?Genome Sequencing of Steroid Producing Bacteria with Illumina Technology.-?Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome.-?RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics.-?Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria.-?Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain MycobacteriumneoaurumNRRL B-3805.-?Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas.-?Selection of Biodegrading Phytosterol Strains.-?Stigmasterol Removal by an Aerobic Treatment System.-?Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione.-??-Sitosterol Bioconversion to Androstenedionein Microtiter Plates.-?Bioconversion of Phytosterols into Androstenedione by Mycobacterium.-?Scale-Up of Phytosterols Bioconversion into Androstenedione.-?Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatisCECT 8331.-?Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD).-?Laboratory-Scale Hydroxylation of Steroids by P450_BM3Variants.-?Obtaining of 11?-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols.-?Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulansand Aspergillus ochraceus.-?Biotransformation of DHEA into 7?, 15?-diOH-DHEA.-?Stabilization of Enzymes by Using Thermophiles.-?Biocatalysis of Steroids with MycobacteriumSp. in Aqueous and Organic Media.-?Downstream Process Synthesis for Microbial Steroids.-?Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC).-?A Ligand-Binding Assay to Measure the Affinity and Specificity of Sterol-Binding Proteins In Vitro.?