Bacterial Protein Secretion Systems -

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Résumé :
Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder.-?Protein Sorting Prediction?.-?Cell Fractionation.-?Defining Lipoprotein Localisation by Fluorescence Microscopy.-?Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate.-?Defining Membrane Protein Localization by Isopycnic Density Gradients.-?Components Sub-cellular Localisation: Cell Surface Exposure.-?Probing Inner Membrane Protein Topology by Proteolysis.-?Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAM^TM).-?Defining Membrane Protein Topology using pho-lac Reporter Fusions.-?In vivo and in vitro Protein-peptidoglycan Interactions.-?Measure of Peptidoglycan Hydrolase Activity.-?Protein-protein Interaction: Bacterial Two-Hybrid.-?Protein-protein Interactions: Yeast two-hybrid.-?Protein-protein Interactions: Cytology Two-hybrid.-?Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial Membranes.-?Protein-protein Interactions: Co-immunoprecipitation.-?Protein-protein Interaction: Tandem Affinity Purification in Bacteria.-?Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes.-?Protein-protein Interactions: Pull-down Assays.-?Protein-protein interactions: Surface Plasmon Resonance.-?Assessing Energy-dependent Protein Conformational Changes in the TonB System.-?Defining Assembly Pathways by Fluorescence Microscopy.-?Large Complexes: Cloning Strategy, Production, and Purification.-?Shearing and Enrichment of Extracellular Type IV Pili.-?Blue Native PAGE Analysis of Bacterial Secretion Complexes.-?In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography.-?Structural Analysis of Protein Complexes by Cryo Electron Microscopy.-?Bacterial Filamentous Appendages Investigated by Solid-state NMR Spectroscopy.-?Identification?of Effectors:? Precipitation of Supernatant Material.-?Energy Requirements for Protein Secretion via the Flagellar type-III Secretion System.-?Screening for Secretion of the Type VI Secretion Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot.-?Effector Translocation: Cya?Reporter Assay.-?Monitoring Effector Translocation with the TEM-1 Beta-lactamase Reporter System.-?Effector Translocation Assay: Differential Solubilization.-?Quantitative Determination of Anti-bacterial Activity during Bacterial Co-culture.?