Gene Synthesis -

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Résumé :
The de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular Biology? series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. ? Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider.

Sommaire:
Section I: Assembly of Oligonucleotides in Synthons?1. Building Block Synthesis using the Polymerase Chain Assembly (PCA) MethodJulie A. Marchand and Jean Peccoud??2. Oligonucleotide Assembly in Yeast to Produce Synthetic DNA FragmentsDaniel G. Gibson ?3. TopDown Real-Time Gene SynthesisMo Chao Huang, Wai Chye Cheong, Hongye Ye, Mo-Huang Li?4. De novo DNA Synthesis using Single-molecule PCRTuval Ben Yehezkel, Gregory Linshiz, and Ehud ShapiroSection II: Synthon Assembly?5. SLIC: A Method for Sequence and Ligation Independent CloningMamie Z. Li and Stephen J. Elledge6. Assembly of Standardized DNA Parts Using BioBrick Ends in E. coliOlivia Ho-Shing, Kin Lau, William Vernon, Todd T. Eckdahl, and A. Malcolm Campbell?7. ?Assembling DNA Fragments by USER FusionNarayana Annaluru, H?lo?se Muller, Sivaprakash Ramalingam, Karthikeyan Kandavelou, Viktoriya London, Sarah M. Richardson, Jessica S. Dymond, Eric M. Cooper, Joel S. Bader, Jef D. Boeke and Srinivasan Chandrasegaran?8. Fusion PCR via Novel Overlap Sequences Kamonchai Cha-aim, Hisashi Hoshida, Tomoaki Fukunaga, and Rinji Akada??9. Using Recombineering to Generate Point Mutations: the Oligonucleotide-based ?Hit & Fix? MethodSuhwan Chang, Stacey Stauffer and Shyam K. Sharan?10. Using Recombineering to Generate Point Mutations: galK-Based Positive-Negative Selection Method Kajal Biswas, Stacey Stauffer and Shyam K. Sharan11. Assembling Large DNA Segments in YeastH?lo?se Muller, Narayana Annaluru, Joy Wu Schwerzmann ,Sarah M. Richardson, Jessica S. Dymond, Eric M. Cooper, Joel S. Bader, Jef D. Boeke, Srinivasan Chandrasegaran?12. Recursive Construction of Perfect DNA Molecules and Libraries from Imperfect Oligonucleotides Gregory Linshiz ??Tuval Ben Yehezkel and Ehud Shapiro?13. Cloning Whole Bacterial Genomes in YeastGwynedd A. Benders??14. Production of Infectious Poliovirus from Synthetic Viral GenomesJeronimo Cello and Steffen MuellerSection III: Software for gene synthesis ?15. In Silico Design of Functional DNA ConstructsAlan Villalobos, Mark Welch, and Jeremy Minshull?16. Using DNAWorks in Designing Oligonucleotides for PCR-Based Gene SynthesisDavid Hoover?17. De Novo Gene Synthesis Design Using TmPrime SoftwareMo-Huang Li, Marcus Bode, Mo Chao Huang, Wai Chye Cheong, Li Shi Lim ??18. Design-A-Gene with GeneDesignSarah M. Richardson, Steffi Liu, Jef D. Boeke, and Joel S. Bader?Section IV: Education and Security 19. Leading a Successful iGEM Team ? Wayne Materi?20. The Build-a-Genome CourseEric M. Cooper, Hel?ise M?ller, Srinivasan Chandrasegaran, Joel Bader, and Jef D. Boeke21. DNA Synthesis SecurityAli Nouri and Christopher F. Chyba?