Germline Development -

. .

Résumé :
The germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation, making these cells amenable to in-depth genomic and mechanistic studies. In Germline Development: Methods and Protocols, expert researchers provide a wide-ranging collection of techniques that can be applied to the study of both the male and female germline.? As the techniques used for isolating and studying male and female germ cells are slightly dissimilar, the different technologies are grouped under two sections in this volume, namely, male germline and female germline.? This collection brings the field of germline developmental studies together such that readers can find, in a single volume, techniques that allow the isolation of gonads with germ cells at different stages of development, the study of the genomics and epigenomics of germ cells, and an examination of the role of single genes in the developmental potential and function of germ cells. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and the avoidance of known pitfalls.? ? Comprehensive and cutting-edge, Germline Development: Methods and Protocols serves as a comprehensive text to individuals, particularly researchers in reproductive and developmental biology, who are interested in studying cells of the germ lineage.

Sommaire:

Part I: Male Germline

? 1. Isolation of Fetal Gonads from Embryos of Timed-Pregnant Mice for Morphological and Molecular Studies

??????????? Yunmin Li, Teruko Taketo, and Yun-Fai Chris Lau

?

??????????? Gurpreet Manku, Monty Mazer, and Martine Culty

??????????? Thomas Garcia and Marie-Claude Hofmann

??????????? Zuping He, Maria Kokkinaki, Jiji Jiang, Wenxian Zeng, Ina Dobrinski, and Martin Dym

??????????? Catherine Boucheron and Vanessa Baxendale

??????????? Yan Wang and Martine Culty

??????????? Tin-Lap Lee, Owen M. Rennert, and Wai-Yee Chan

??????????? Alan Lap-Yin Pang

??????????? Tin-Lap Lee, Amy Xiao, and Owen M. Rennert

??????????? Hoi-Hung Cheung, Tin-Lap Lee, Owen M. Rennert, and Wai-Yee Chan

??????????? Alexander I. Agoulnik, Zaohua Huang, and Lydia Ferguson

??????????? Shyamal K. Roy, Cheng Wang, Anindit Mukherjee, and Prabuddha Chakraborty

??????????? Teruko Taketo

??????????? Ye Yuan and Rebecca L. Krisher

??????????? Andrew J. Childs and Richard A. Anderson

??????????? Chang Liu, Melissa Paczkowski, Manal Othman, and Humphrey Hung-Chang Yao

??????????? Hisato Kobayashi and Tomohiro Kono

??????????? Eric Antoniou and Robert Taft