Plant Developmental Biology -

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Résumé :
Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. Long before Mendel discovered the laws of heritab- ity and Darwin developed his theory on evolution, the affection for ornamental plants led people to select alleles that establish novel plant forms. Today, plant developmental biology tries to discover the mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant?s life. Although the underlying processes of cell proliferation and differentiation are similar in plants and a- mals, plants are different because their development is usually open, and its outcome is not the faithful repetition of a general plan but is strongly in?uenced by environm- tal conditions. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge. In part, this progress was enabled by the advance of powerful molecular tools for a few model species, most importantly Arabidopsis. This volume of the Methods in Molecular Biology series provides a collection of protocols for many of the common experimental approaches in plant developmental bi- ogy. All chapters are written in the same format as that used in the Methods in Molecular TM Biology series. Each chapter opens with a description of the basic theory behind the method being described.

Sommaire:
Plant Developmental Biology Edited by Lars Hennig and Claudia K?hler Contents 1. Growth protocols for model plants in developmental biology Lars Hennig 2. Grafting as a research tool Colin G. N. Turnbull 3. Viral-induced gene silencing as a reverse genetics tool to study gene function Steven Bernacki, Mansour Karimi, Pierre Hilson and Niki Robertson 4. The CRE/lox system as a tool for developmental studies at the cell and tissue level Guy Wachsman and Renze Heidstra 5. Inducible gene expression systems for plants Lorenzo Borghi 6. Trichome development in Arabidopsis Joachim F. Uhrig and Martin H?lskamp 7. Phenotyping the development of leaf area in Arabidopsis thaliana Sarah J. Cookson, Olivier Turc, Catherine Massonnet and Christine Granier 8. Analyzing shoot apical meristem development Cristel Carles, Chan Man Ha, Ji Hyung Jun, Elisa Fiume and Jennifer C. Fletcher 9. Analyzing floral meristem development Elisa Fiume, Helena R. Pires, Jin Sun Kim and Jennifer C. Fletcher 10. Female gametophytic mutants: Diagnosis and characterization Ronny V?lz and Rita Gro?-Hardt 11. Pollen tube development Mark A. Johnson and Benedikt Kost 12. Analysis of root meristem size development Serena Perilli and Sabrina Sabatini 13. Phenotypic characterization of photomorphogenic responses during plant development Thomas Kretsch 14. Kinematic analysis of cell division and expansion Bart Rymen, Frederik Coppens, Stijn Dhondt, Fabio Fiorani and Gerrit T.S. Beemster 15. Flowering time control Yvonne M?ller-Steinbach, Cristina Alexandre and Lars Hennig 16. mRNA detection by whole mount in situ hybridization WISH or sectioned tissue in situ hybridzation SISH in Arabidopsis Yvonne Stahl and R?diger Simon 17. Immunolocalization of proteins in plants Michael Sauer and Ji?? Friml 18. Detection of small non-coding RNAs Tamas Dalmay 19. Quantitative real time PCR in plant developmental biology Vivien Exner 20. Luciferase and green fluorescent protein reporter genes as tools todetermine protein abundance and intracellular dynamics Andr?s Viczi?n and Stefan Kircher 21. Fluorescence-activated cell sorting in plant developmental biology Anjali S. Iyer-Pascuzzi and Philip N. Benfey 22. Laser microdissection of paraffin embedded plant tissues for transcript profiling Robert C. Day 23. Utilizing bimolecular fluorescence complementation BiFC to assay protein-protein interaction in plants Nir Ohad and Shaul Yalovsky 24. The split luciferase complementation assay Naohiro Kato and Jason Jones 25. Co-immunoprecipitation and protein blots Erika Isono and Claus Schwechheimer 26. Probing protein-protein interactions with FRET-FLIM Christoph B?cherl, Jos? Aker, Sacco de Vries, Jan Willem Borst 27. Plant chromatin immunoprecipitation Corina B. R. Villar and Claudia K?hler 28. Immunocytological analysis of chromatin in isolated nuclei Penka Pavlova, Federico Tessadori, Hans J. de Jong and Paul Fransz 29. Bisulphite sequencing of plant genomic DNA Ernst Aichinger and Claudia K?hler