Diagnostic Virology Protocols -

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Résumé :
A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.

Sommaire:
Rapid Detection of Adenovirus from Fecal Specimens Tanvir Tabish, Alan Warnes, and Stuart Clark Alphaviruses John T. Roehrig, Teresa M. Brown, Alison J. Johnson, Nick Karabatsos, Denise A. Martin, Carl J. Mitchell, and Roger S. Nasci Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR Xi Jiang and David O. Matson Enteroviruses and Rhinoviruses Peter Muir Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction Dennis W. Trent, Gwong-Jen Chang, A. Vance Vorndam, and Robert S. Lanciotti Hepatitis Viruses Girish J. Kotwal Herpesviruses Mark J. Espy, P. Shawn Mitchell, David H. Persing, and Thomas F. Smith Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper Sharon Cassol, Stanley Read, Bruce G. Weniger, Richard Pilon, Barbara Leung, and Theresa Mo PCR for the Detection of Influenza Viruses in Clinical Material Lyssaviruses: Special Emphasis on Rabies Virus Herv? Bourhy Methods for Detecting Anti-Measles, Mumps, and Rubella Virus Antibodies Ilkka Julkunen, Irja Davidkin, and Christian Oker-Blom Papillomaviruses Sandra H. Kirk and David T. Y. Liu Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization Karen E. Hicks, Stuart Beard, Bernard J. Cohen, and Jonathan P. Clewley Polioviruses: Concurrent Serotyping and Intratypic Differentiation of Polioviruses David J. Wood Poxviruses Hermann Meyer, Susan L. Ropp, and Joseph J. Esposito Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections G. L. Toms Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis Lennart Svensson Detection of PCR-Amplified Sandfly Fever Sicilian Virus RNAby Colorimetric Enzymatic Immunoassay M. Sofi Ibrahim and Connie S. Schmaljohn Time-Resolved Fluorescence Pekka Halonen and Timo L?vgren Enzyme-Linked Immunosorbent Assays David M. Kemeny Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology Joakim Lundeberg, Jacob Odeberg, and Mathias Uhl?n Robotic Analyzers Dale R. Pfost and Richard G. Keightley The Detection of Enteroviruses in Water and Associated Materials Using the Polymerase Chain Reaction Peter Wyn-Jones and Jane Sellwood Recombinant Antigens in Viral Diagnosis John R. Stephenson and Alan Warnes NASBA: A Method for Nucleic Acid Diagnostics Dianne van Strijp and Pierre van Aarle Oligonucleotides Stina Syrj?nen Index