Cell Cycle Control -

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Résumé :
This collection of cutting-edge techniques for the study of the eukaryotic cell cycle and its key regulatory molecules includes overviews of cell cycle regulatory mechanisms in many major research organisms. Described in step-by-step detail, these readily reproducible methods enable fundamental research on well-defined cell cycle regulators-and those more recently defined-in yeasts, bacteria, plants, Drosophila, Xenopus, and mammals. The book offers all cell researchers indispensable techniques essential to understanding how normal cells divide and how this is altered in disease.

Sommaire:
Part I. Overviews of Cell Cycle Control in Different Organisms Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts Tim Humphrey and Amanda Pearce The Plant Cell Cycle: An Overview John H. Doonan The C. elegans Cell Cycle: Overview of Molecules and Mechanisms Sander van den Heuvel Developmental Control of Growth and Cell Cycle Progression in Drosophila Lisa Swanhart, Jeremy Kupsco, and Robert J. Duronio The Xenopus Cell Cycle: An Overview Anna Philpott and P. Renee Yew The Mammalian Cell Cycle: An Overvew Jane V. Harper and Gavin Brooks Part II. Cell Cycle Study Methods and Techniques Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle Jane V. Harper Mapping Origins of DNA Replication in Eukaryotes Susan A. Gerbi In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast Stephen E. Kearsey, Lydia Brimage, Mandana Namdar, Emma Ralph, and Xiaowen Yang Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short Hairpin RNAs Peter D. Adams The Cell Cycle and Virus Infection Stevan R. Emmett, Brian Dove, Laura Mahoney, Torsten Wurm, and Julian A. Hiscox Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies Julie Welburn and Jane Endicott Part III. Detection and Measurement of Cell Cycle Molecules E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression Ludger Hauck and R?diger von Harsdorf Forkhead (FOX) Transcription Factors and the Cell Cycle: Measurement of DNA Binding by FoxO and FoxM Transcription Factors Katrina A. Bicknell Measurement of Geminin Activity in Xenopus Egg Extracts Thomas J. McGarry CDK-ActivatingKinases: Detection and Activity Measurements St?phane Larochelle and Robert P. Fisher Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements Gavin Brooks Measurement of Wee Kinase Activity Paul R. Mueller and Walter F. Leise III CDC25 Dual-Specificity Protein Phosphatases: Detection and Activity Measurements Sorab N. Dalal and Melanie Volkening Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1 Carmela Palermo and Nancy C. Walworth Polo-Like Kinase-1: Activity Measurement and RNAi-Mediated Knockdown Marcel A. T. M. van Vugt and Ren? H. Medema The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells Claire Ditchfield, Nicholas Keen, and Stephen S. Taylor Purification of the Ndc80 Kinetochore Subcomplex From Xenopus Eggs Mark L. McCleland and P. Todd Stukenberg Index