Amino Acid Analysis Protocols -

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Résumé :
A collection of classic and cutting-edge techniques of high utility in answering specific biological questions about amino acids. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Each method is described in step-by-step detail to ensure successful experimental results and emphasizes sample preparation, particularly the collection and storage of bodily fluids. Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

Sommaire:
Amino Acid Analysis: An Overview, Margaret I. Tyler. Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory, Frank D. Macchi, Felicity J. Shen, Rodney G. Keck, and Reed J. Harris. Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges for Amino Acid Analysis, Li Zhang and Nancy Denslow. Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate Steven A. Cohen. Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-l-Alanine Amide (Marfey's Reagent), Sunil Kochhar, Barbara Mouratou, and Philipp Christen. The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection, C. David Forster and Charles A. Marsden. Anion Exchange Chromatography and Intergrated Amperometric Detection of Amino Acids, Petr Jandik, Christopher Pohl, Victor Barreto, and Nebojsa Avdalovic. Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane, Noriko Shindo, Tsutomu Fujimura, Saiko Kazuno, and Kimie Murayama. Capillary Gas Chromatographic Analysis of Protein and Nonprotein Amino Acids in Biological Samples, Hiroyuki Kataoka, Sayuri Matsumura, Shigeo Yamamoto, and Masami Makita. Measurement of Blood Plasma Amino Acids in Ultrafiltrates by High-Performance Liquid Chromatography with Automatic Precolumn O-Phthaldialdehyde Derivatization, Hua Liu. Determination of Amino Acids in Foods by Reversed-Phase High-Performance Liquid Chromatography with New Precolumn Derivatives, Butylthiocarbamyl, and Benzylthiocarbamyl Derivatives Compared to the Phenylthiocarbamyl Derivative and Ion Exchange Chromatography, Kang-Lyung Woo. Amino Acid Measurement in Body Fluids Using PITC Derivatives, Roy A. Sherwood. Determination of Proteins, Phosphatidylethanolamine, and Phosphatidylserine in Lipid-Rich Materials by Analysis. of Phenylthiocarbamyl Derivatives, Margareta Stark and Jan Johansson. Analysis of O-Phosphoamino Acids in Biological Samples by Gas Chromatography with Flame Photometric Detection, Hiroyuki Kataoka, Norihisa Sakiyama, Yukizo Ueno, Kiyohiko Nakai, and Masami Makita. Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection, Hiroyuki Kataoka, Kiyomi Takagi, Hirofumi Tanaka, and Masami Makita. Capillary Electrophoretic Determination of 4-Hydroxyproline, Qingyi Chu and Michael Zeece. Total Plasma Homocysteine Analysis by HPLC with SBD-F Precolumn Derivatization, Isabella Fermo and Rita Paroni. Determination of Early Glycation Products by Mass Spectrometry and Quantification of Glycation Mediated Protein Crosslinks by the Incorporation of [14C]lysine into Proteins, Malladi Prabhakaram, Beryl J. Ortwerth, and Jean B. Smith. Index.