Type 3 Secretion Systems -
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Présentation Type 3 Secretion Systems de Format Broché
- Livre Encyclopédies, Dictionnaires
Résumé : Introduction to Type III Secretion Systems.-?Site-Directed Mutagenesis and its Application in Studying the Interactions of T3S Components.-?Blue Native Protein Electrophoresis to Study the T3S System using Yersinia pestisas a Model.- In Vivo Photo-Cross-Linking to Study T3S Interactions Demonstrated using the Yersinia pestisT3S System.-?Isolation of Type III Secretion System Needle Complexes by Shearing.-?Use of Transcriptional Control to Increase Expression and Secretion of Heterologous Proteins in T3S Systems.-?Characterization of Type Three Secretion System Translocator Interactions with Phospholipid Membranes.-?Analysis of Type III Secretion System Secreted Proteins.-?Fractionation Techniques to Examine Effector Translocation.- Measurement of Effector Protein Translocation using Phosphorylatable Epitope Tags and Phospho-Specific Antibodies.-?A TAL-Based Reporter Assay for Monitoring Type III-Dependent Protein Translocationin Xanthomonas.-?Subcellular Localization of Pseudomonas syringaepv. tomatoEffector Proteins in Plants.-?A Method for Characterizing the Type III Secretion System's Contribution to Pathogenesis: Homologous Recombination to Generate Yersinia pestisType III Secretion System Mutants.-?Detecting Immune Responses to Type III Secretion Systems.-?Recombinant Expression and Purification of the Shigella Translocator IpaB.-?Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins.-?Mouse Immunization with Purified Needle Proteins from Type III Secretion Systems and the Characterization of the Immune Response to these Proteins.- Identification of the Targets of Type III Secretion System Inhibitors.-?Detection of Protein Interactions in T3S Systems using Yeast 2-Hybrid Analysis.
Biographie:
use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems...
Sommaire:
This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components...
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