CCN Proteins -

. .

Résumé :

The CCN Proteins: An Overview.-?Gene Expression Analysis of CCN Proteins: Whole Mount In Situ Hybridization Of CCN2 in Developing Calcified Tissues?.-?Expression of CCN Genes and Proteins in Human Skin: Methods And Protocols.-?Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells.-?Western Blotting Analysis of CCN Proteins.-??Immunohistochemical Analysis of CCN Proteins.-?Analysis of CCN Expression by Immunofluorescence on Skin Cells, Skin, and Reconstructed Epidermis.-??Production of Recombinant CCN2 Protein in Escherichia. Coli.-??Production Of Recombinant CCN Proteins By Brevibacillus Choshinensis.-?Production Of Recombinant CCN2 Protein By Mammalian Cells.-?In Vitro Transfection with and Expression of CCN Family of Genes.-?Preparation of Module Specific Antibodies against CCN2.-?ELISA of CCN Proteins in Body Fluids Including Serum And Plasma.-?Analysis of Signaling Pathways Activated by CCN Proteins.-?Protocols for Screening for Binding Partners of CCN Proteins: Yeast Two- Hybrid System.-?Protocols For Screening Peptide Motifs Binding To CCN Proteins.-?Evaluation Of Molecular Interaction Between CCN Proteins and its Binding Partners By Surface Plasmon Resonance (SPR).-??Promoter Analysis of CCN Genes.-?Analysis Of Posttranscriptional Regulation Of CCN Genes.-??Protein Imaging of CCN2 and CCN3 in Living Cells.-??Cell Biological Assays for Measuring Chondrogenic Activities of CCN Proteins.-??Protocol for Measuring Angiogenic Activities of CCN Proteins.-?Cell Biological Assays for Measuring Odontogenic Activities of CCN Proteins.-?Separation and Enrichment of Haematopoietic Stem Cells for CCN Studies.-?In Vivo Evaluation of Cartilage Regenerative Effects of CCN Proteins.-?Gene Expression Analysis of CCN Proteins in Bone Under Mechanical Stress.-?The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of CCN3 Transgenic and Knockout Mice.-?Design and Analysis of CCN Gene Activity Using CCN Knockout Mice Containing Lacz Reporters.-?Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches.-?Construction and Analysis of an Allelic Series of CCN1 Knockin Mice.-??Production and Analysis of Conditional KO Mice of CCN2 in Kidney.-?Generation And Analysis Of Cartilage-Specific CCN2 Overexpression In Transgenic Mice.-?Analysis of Transcytosis of CCN2 by Chondrocytes.-?CCN2 in Skin Fibrosis.-?Studying the CCN Proteins in Fibrosis.-?Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases - Kidney Fibrosis.-?Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases - Liver Fibrosis.-?Cellular or Exosomal Micrornas Associated with CCN Gene Expression in Liver Fibrosis.-?TGF-?1 sndCCN2-Stimulated Sirius Red Assay for Collagen Accumulation in Cultured Cells.-??CCN Detection of Cancer Tissues by Immunohistochemistry Staining.-?Gene Expression Analysis of CCN Proteins: Detection of CCN1 and CCN5 MRNA in Human Cancer Samples using a Modified In Situ Hybridization Technique.-?Analysis of Pathological Activities of CCN Proteins in Bone Metastasis.-?Analysis of Pathological Activities of CCN2/CTGF in Muscle Dystrophy.-??Method for Analysis of Matrix Degradation by CCN2 through the MMP/TIMP System.-?An Analysis of Pathological Activities of CCN Proteins in Joint Disorders?Mechanical Stretch-Mediated CCN2 Expression in Cultured Meniscus Cells?.-??Analysis of CCN Protein Expression and Activities in Vasoproliferative Retinopathies.?

...

Sommaire:

The CCN Proteins: An Overview.-?Gene Expression Analysis of CCN Proteins: Whole Mount In Situ Hybridization Of CCN2 in Developing Calcified Tissues?.-?Expression of CCN Genes and Proteins in Human Skin: Methods And Protocols.-?Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells.-?Western Blotting Analysis of CCN Proteins.-??Immunohistochemical Analysis of CCN Proteins.-?Analysis of CCN Expression by Immunofluorescence on Skin Cells, Skin, and Reconstructed Epidermis.-??Production of Recombinant CCN2 Protein in Escherichia. Coli.-? ?Production Of Recombinant CCN Proteins By Brevibacillus Choshinensis.-? Production Of Recombinant CCN2 Protein By Mammalian Cells.-?In Vitro Transfection with and Expression of CCN Family of Genes.-?Preparation of Module Specific Antibodies against CCN2.-?ELISA of CCN Proteins in Body Fluids Including Serum And Plasma.-?Analysis of Signaling Pathways Activated by CCN Proteins.-?Protocols for Screening for Binding Partners of CCN Proteins: Yeast Two- Hybrid System.-?Protocols For Screening Peptide Motifs Binding To CCN Proteins.-?Evaluation Of Molecular Interaction Between CCN Proteins and its Binding Partners By Surface Plasmon Resonance (SPR).-??Promoter Analysis of CCN Genes.-?Analysis Of Posttranscriptional Regulation Of CCN Genes.-??Protein Imaging of CCN2 and CCN3 in Living Cells.-??Cell Biological Assays for Measuring Chondrogenic Activities of CCN Proteins.-??Protocol for Measuring Angiogenic Activities of CCN Proteins.-?Cell Biological Assays for Measuring Odontogenic Activities of CCN Proteins.-?Separation and Enrichment of Haematopoietic Stem Cells for CCN Studies.-?In Vivo Evaluation of Cartilage Regenerative Effects of CCN Proteins.-?Gene Expression Analysis of CCN Proteins in Bone Under Mechanical Stress.-?The Bone Regeneration Model and Primary Osteoblastic Cell Culture Used in the Analysis of CCN3 Transgenic and Knockout Mice.-?Design and Analysis of CCN Gene Activity Using CCN Knockout Mice Containing Lacz Reporters.-?Analysis of CCN4 Function in Osteogenic and Osteoclastic Cells Using Gain and Loss of Function Approaches.-?Construction and Analysis of an Allelic Series of CCN1 Knockin Mice.-??Production and Analysis of Conditional KO Mice of CCN2 in Kidney.-?Generation And Analysis Of Cartilage-Specific CCN2 Overexpression In Transgenic Mice.-?Analysis of Transcytosis of CCN2 by Chondrocytes.-?CCN2 in Skin Fibrosis.-?Studying the CCN Proteins in Fibrosis.-?Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases - Kidney Fibrosis.-?Analysis of Pathological Activities of CCN Proteins in Fibrotic Diseases - Liver Fibrosis.-?Cellular or Exosomal Micrornas Associated with CCN Gene Expression in Liver Fibrosis.-?TGF-?1 sndCCN2-Stimulated Sirius Red Assay for Collagen Accumulation in Cultured Cells.-??CCN Detection of Cancer Tissues by Immunohistochemistry Staining.-?Gene Expression Analysis of CCN Proteins: Detection of CCN1 and CCN5 MRNA in Human Cancer Samples using a Modified In Situ Hybridization Technique.-?Analysis of Pathological Activities of CCN Proteins in Bone Metastasis.-?Analysis of Pathological Activities of CCN2/CTGF in Muscle Dystrophy.-??Method for Analysis of Matrix Degradation by CCN2 through the MMP/TIMP System.-?An Analysis of Pathological Activities of CCN Proteins in Joint Disorders?Mechanical Stretch-Mediated CCN2 Expression in Cultured Meniscus Cells?.-??Analysis of CCN Protein Expression and Activities in Vasoproliferative Retinopathies.?

...