Yeast Protocols -

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Résumé :
In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.

Sommaire:
Basic Investigations in Saccharomyces cerevisiae Brendan P. G. Curran and Virginia Bugeja Isolation of Nucleic Acids Michelle Hanna and Wei Xiao Purification of Yeast Peroxisomes Ben Distel and Astrid Kragt Isolation of Yeast Plasma Membranes Barry Panaretou and Peter Piper Isolation of Yeast Mitochondria Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott Extraction of Yeast Lipids Roger Schneiter and G?nther Daum Two-Dimensional Gel Electrophoresis of Total Yeast Proteins H?lian Boucherie and Christelle Monribot-Espagne Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes Laura Maringele and David Lydall. Analysis of Yeast Lipids, Roger Schneiter and G?nther Daum Yeast Fluorescence Microscopy Jir? Hasek Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe Michelle A. Alting-Mees, Eddy P. Risseeuw, Enwu Liu, Michel Desautels, William A. Crosby, and Sean M. Hemmingsen Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method R. Daniel Gietz and Robin A. Woods Mutagenesis Leslie Barbour, Michelle Hanna, and Wei Xiao Gene Disruption in the Budding Yeast Saccharomyces cerevisiae Johannes H. Hegemann, Ulrich G?ldener, and Gabriele J. K?hler Inducible Degron and Its Application to Creating Conditional Mutants R. J?rgen Dohmen Synthetic Lethal Screen Leslie Barbour and Wei Xiao Synthetic Genetic Array Analysis in Saccharomyces cerevisiae Amy Hin Yan Tong and Charles Boone Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates Alain T. Dandjinou, Michel Larriv?e, Ralf E. Wellinger, and Raymund J. Wellinger Chromatin Assembly in a Crude Fraction From Yeast Cells Karen M.Robinson and Michael C. Schultz Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast Elena Ezhkova and William P. Tansey Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning Zhengjian Zhang and Joseph C. Reese Study of Transcriptional Regulation Using a Reporter Gene Assay Yu Fu and Wei Xiao Assessing Telomeric Phenotypes Catherine LeBel, Michel Larriv?e, Amadou Bah, Nancy Laterreur, Nancy L?vesque, and Raymund J. Wellinger Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast Zhigang Wang Production of Heterologous Proteins in Yeast With the Aid of the Hsp150D Carrier Marja Makarow, Anna-Liisa H?nninen, Taina Suntio, and Ricardo Nunes Bastos Yeast Two-Hybrid System Screening R. Daniel Gietz Import of Precursor Proteins Into Isolated Yeast Mitochondria Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling Index