West Nile Virus -

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Résumé :
This volume is a collection of research methods, techniques, and approaches to investigate the molecular biology of West Nile Virus (WNV). Chapters in the book cover many facets of WNV, such as propagation and titration of WNV in vero cells, examination of WNV neuroinvasion and neuropathogenesis in the central nervous system of a murine model, field surveillance methods for WNV, and in vitro and in vivo blood-brain barrier models to study WNV pathogenesis. A brief introduction, along with a discussion on WNV laboratory safety are also included. Written in the highly successful Methods in Molecular Biology series format, chapters includes introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, West Nile Virus will interest virologists, molecular biologists, microbiologists, and other scientists working with WNV. This book is a comprehensive guide for those conducting research in the field of WNV biology, including life cycles, pathogenesis, impact of infection, and possible treatment/prevention development.

Sommaire:
1.?A Brief Review of West Nile Virus BiologyBerlin Londono-Renteria and Tonya M. Colpitts2.??Overview of West Nile Virus Transmission and EpidemiologyAndrea Troupin and Tonya M. Colpitts3.?Propagation and Titration of West Nile Virus on Vero CellsAlexander J. McAuley and David W. C. Beasley4.?Generating West Nile Virus from an Infectious CloneRianna Vandergasst and Brenda L. Frederickson5.?Functional Analysis of West Nile Virus Proteins in Human CellsPakieli H. Kaufusi, Alanna Tseng, and Vivek R. Nerurkar6.?Lessons from the Murine Models of West Nile Virus InfectionBrenna McGruder, Vandana Saxena, and Tian Wang7.?Exploration of West Nile Virus Infection in Mouse ModelsPenghua Wang8.?Examination of West Nile Virus Neuroinvasion and Neuropathogenesis in the Central Nervous System of a Murine ModelHameeda Sultana9.? In Vitro and In Vivo Blood-Brain Barrier Models to Study West Nile Virus PathogenesisMukesh Kumar and Vivek R. Nerurkar10.?Methodology for Identifying Host Factors Involved in West Nile Virus InfectionManoj N. Krishnan11.?ELISA and Neutralization Methods to Measure Anti-West Nile Virus Antibody ResponsesBrian M. Friedrich and David W. C. Beasley12.?Safe Handling of West Nile Virus in the InsectaryDexter R. McKellar and Michael J. Conway13.?Techniques for Experimental Infection of Mosquitoes with West Nile VirusYang Liu and Gong Cheng14.??Field Surveillance Methods for West Nile VirusSamuel B. Jameson, and Dawn M. Wesson15.?Detection Protocols for West Nile Virus in Mosquitoes, Birds, and Non-Human MammalsElizabeth B. Kauffman, Mary A. Franke, and Laura D. Kramer16.?Field Methods and Sample Collection Techniques for the Surveillance of West Nile Virus in Avian HostsSarah S. Wheeler, Walter M. Boyce, and William K. Reisen?17.?Statistical Tools for the Interpretation of Enzootic West Nile Virus Transmission DynamicsKevin A. Caillou?t and Suzanne Robertson18.?Role of Immune Aging in Susceptibility to West Nile VirusYi Yao and Ruth R. Montgomery19.?An Overview of Current Approaches Toward the Treatment and Prevention of West Nile Virus InfectionDhiraj Acharya and Fengwei Bai