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Présentation The Mitotic Spindle Format Broché
- Livre Physique - Chimie
Résumé : Using Fluorescence Microscopy to Study Mitosis.- Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation.- Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts.-?Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro.-Imaging and Quantifying the Dynamics of ?-Tubulin at Microtubule Minus Ends in Mitotic Spindles.- Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic XenopusEgg Extracts and TIRF Microscopy.-?Encapsulation of Xenopus Egg and Embryo Extract Spindle Assembly Reactions?in Synthetic Cell-Like Compartments with Tunable Size.- In VitroKinetochore Assembly.-?Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes.-?Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells.- Studying Kinetochores In Vivo Using FLIM-FRET.-?Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies.-?A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion.-?Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation.-?Analyzing Spindle Positioning Dynamics in Cultured Cells.-?Quantification of Mitotic Chromosome Alignment.- Imaging Mitosis in the Moss Physcomitrella Patens.-?Small Molecule Approach to Study the Function of Mitotic Kinesins.-?Identification and Characterization of Mitotic Spindle-Localized Transcripts.-?Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization.- Studying Kinetochore Kinases.- Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics.-?Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer.-?Generation and Purification of Tetraploid Cells.-?Anti-Microtubule Drugs.
Sommaire: Using Fluorescence Microscopy to Study Mitosis.- Using Photoactivatable GFP to Study Microtubule Dynamics and Chromosome Segregation.- Purification and Fluorescent Labeling of Tubulin from Xenopus Laevis Egg Extracts.-?Measuring the Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro.- Imaging and Quantifying the Dynamics of ?-Tubulin at Microtubule Minus Ends in Mitotic Spindles.- Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy.-?Encapsulation of Egg and Embryo Extract Spindle Assembly Reactions?in Synthetic Cell-Like Compartments with Tunable Size.- In Vitro Kinetochore Assembly.-?Biochemical and Structural Analysis of Kinetochore Histone-Fold Complexes.-?Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells.- Studying Kinetochores In Vivo Using FLIM-FRET.-?Purification of Fluorescently Labeled Saccharomyces Cerevisiae Spindle Pole Bodies.-?A Cell-Free System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome Conversion.-?Assays to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation.-?Analyzing Spindle Positioning Dynamics in Cultured Cells.-?Quantification of Mitotic Chromosome Alignment.- Imaging Mitosis in the Moss Physcomitrella Patens.-? Small Molecule Approach to Study the Function of Mitotic Kinesins.-?Identification and Characterization of Mitotic Spindle-Localized Transcripts.-?Probing Mitosis by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible Dimerization.- Studying Kinetochore Kinases.- Engineering and Functional Analysis of Mitotic Kinases through Chemical Genetics.-?Using Cell Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer.-?Generation and Purification of Tetraploid Cells.-?Anti-Microtubule Drugs.
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