Plant Genetic Engineering - Grierson, D.

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Sommaire:
1 Plant gene structure and expression.- 1.1 Introduction.- 1.2 Protein-coding genes.- 1.3 Regulation of plant gene expression.- 1.4 Translational control.- 1.5 Differential expression.- 1.6 RNA-coding genes.- 1.7 RNA genes transcribed by RNA polymerase I.- 1.8 RNA genes transcribed by RNA polymerase II.- 1.9 RNA genes transcribed by RNA polymerase III.- References.- 2 Gene transfer to plants.- 2.1 Introduction.- 2.2 Vectors based on the Agrobacterium Ti plasmid.- 2.3 Physical DNA delivery methods.- 2.4 Uses of gene transfer technology.- References.- 3 Developing herbicide resistance in crops by gene transfer technology.- 3.1 Introduction.- 3.2 Modification of the target of herbicide action.- 3.3 Detoxification or degradation of the herbicide.- 3.4 Perspectives.- References.- 4 Genetic engineering of plants for insect resistance.- 4.1 Introduction.- 4.2 Defensive methods used by plants against insect attack.- 4.3 Insecticidal compounds from other sources.- 4.4 Constraints on the genetic engineering of plants for insect resistance.- 4.5 The production of insect-resistant transgenic plants: two case studies.- 4.6 Future prospects.- References.- 5 Virus-resistant plants.- 5.1 Introduction.- 5.2 Basic concepts of resistance to plant virus infection.- 5.3 Key features of the infection cycles of positive-strand RNA plant viruses.- 5.4 Molecular bases and exploitation of naturally occurring virus resistance genes.- 5.5 Construction and expression of artificial resistance genes in transgenic plants.- 5.6 Future prospects.- References.- 6 Targeting of proteins to chloroplasts and mitochondria.- 6.1 Introduction.- 6.2 Structure and biogenesis of chloroplasts.- 6.3 Import of stromal proteins.- 6.4 Transport of proteins into the thylakoid system.- 6.5 Import of proteins into the envelope membranes.- 6.6 Transport of proteins into mitochondria.- References.- 7 Protein transport and targeting within the endomembrane system of plants.- 7.1 Introduction.- 7.2 Biogenesis of endomembrane compartments.- 7.3 Mechanisms of protein transport.- 7.4 Mechanisms of protein sorting.- 7.5 Glycosylation and post-translational modification.- References.- 8 Identification and characterisation of tissue-specific genes from flowers.- 8.1 Introduction.- 8.2 Identification of flower-specific genes.- 8.3 Characterisation of gene expression in floral organs.- 8.4 Tissue and cell specificity of gene expression in flowers.- 8.5 Discussion.- References.