Exocytosis Methods - Peter Thorn

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Résumé :
Bringing together techniques and methods currently being applied to the study of exocytosis, Exocytosis Methods collects chapters from experts in the field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission.? The book begins with a section covering a range of techniques being applied to the study of single-vesicle fusion events, which are key in order to gain insight into the final steps of vesicle fusion.? The volume continues with several model systems that are being employed to unravel the complexities of exocytosis.? Written for the Neuromethods series, chapters included in this work present the kind of detail and vital implementation advice that leads to successful results. ? Practical and authoritative, Exocytosis Methods seeks to promote the advent of new methods in microscopy and the development of new preparations, which would doubtlessly lead to many new and exciting discoveries in this field.

Sommaire:

Solution Single-Vesicle Fusion Assay by Single-Molecule Alternating Laser Excitation.- Imaging the Stages of Exocytosis in Epithelial Type II Pneumocytes.- Carbon-Fibre Amperometry in the Study of Exocytosis.- Imaging of Insulin Exocytosis from Pancreatic Beta Cells.- Functional, Quantitative, and Super-Resolution Imaging and Spectroscopic Approaches for Studying Exocytosis.- Electrophysiologic Measurements of Membrane Capacitance in Hormone-Secreting Cells.- Measuring Exocytosis in Endocrine Tissue Slices.- Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Live Rodents.- Rapid Analysis of Synaptic Vesicle Endocytosis in Synaptosomes.- Isolated Neurohypophysial Terminals: Model for Depolarization-Secretion Coupling.- The Sea Urchin Egg and Cortical Vesicles as Model Systems to Dissect the Fast, Ca²⁺-Triggered Steps of Regulated Exocytosis.